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2013 Fiscal Year Final Research Report

Analysis of mitochondrial morphology and movement

Research Project

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Project/Area Number 23570232
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionRikkyo University (2012-2013)
Kyushu University (2011)

Principal Investigator

OKA Toshihiko  立教大学, 理学部, 教授 (40263321)

Project Period (FY) 2011 – 2013
Keywordsミトコンドリア
Research Abstract

An RNA interference screen using nematode led us to identification of a novel kinasin KLP6 involved in mitochondrial morphology and movement. The dominant-negative KLP6 induced abnormal mitochondrial morphology in HeLa cells. Furthermore, anterograde transport of mitochondria in the neurites of differentiated neuro2a cells was significantly reduced when the dominant negative form was overexpressed, indicating that KLP6 regulates intracellular transport of mitochondria.
Downregulation of an inner membrane protein LETM1 induces mitochondrial swelling and fragmentation. Moreover, cristae structure clearly disappeared in the LETM1-knockdown cells. To investigate the LETM1 functions, we attempted to in vitro reconstitute membrane invagination using LETM1 recombinant protein and artificial liposome. This is a good tool to analyze the roles of LETM1 on the formation of mitochondrial inner membrane structures.

  • Research Products

    (6 results)

All 2014 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Regulation and physiologic functions of GTPases in mitochondrial fusion and fission in mammals、Antioxid2012

    • Author(s)
      Ishihara, N., H. Otera, T. Oka, and K. Mihara.
    • Journal Title

      Redox Signal

      Volume: 19 Pages: 389-399

    • DOI

      10.1089/ars.2012.4830

  • [Journal Article] KLP6, a newly identified kinesin that regulates morphology and transport of mitochondria in neuronal cells2011

    • Author(s)
      Tanaka, K., Y. Sugiura, R. Ichishita, K. Mihara and T. Oka.
    • Journal Title

      J. Cell Sci.

      Volume: 124 Pages: 2457-2465

    • Peer Reviewed
  • [Presentation] オルガネラ形態の機能的意義2014

    • Author(s)
      岡敏彦
    • Organizer
      第1回生命分子科学研究会
    • Place of Presentation
      北海道
    • Year and Date
      20140317-19
  • [Presentation] Formation of Cristae Structure in Mammals2013

    • Author(s)
      Toshihiko Oka.
    • Organizer
      The 4^<th> International Symposium on Dynamics of Mitochondria from Molecular Mechanisms to Physiological Functions and Diseases
    • Place of Presentation
      沖縄
    • Year and Date
      20131028-1101
  • [Presentation] ミトコンドリア形態とクリステ膜構造の形成機構2012

    • Author(s)
      岡敏彦
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡
    • Year and Date
      20121214-16
  • [Remarks]

    • URL

      http://www2.rikkyo.ac.jp/web/oka_lab/index.html

URL: 

Published: 2015-07-16  

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