Characterization of a Escherichia coli strain that evolved in an experimental coevolution system

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570268
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Hirosaki University
• Principal Investigator: KASHIWAGI AKIKO 弘前大学, 農学生命科学部, 准教授 (40362652)
• Project Period (FY): 2011 – 2013
• Keywords: 実験進化学 / バクテリオファージ / 大腸菌 / 共進化

Research Abstract

To examine the ongoing changes driven by host-parasite interactions, I have constructed a coevolution model consisting of Escherichia coli and the bacteriophage Qb. Whole genome analysis indicated that E.coli showed fixation of two mutations (in traQ anc csdA) faster than in sole E.coli experimental evolution.
To elucidate the mechanism underlying this coexistence, I investigated how a mutation in TraQ changed the physiological state of E. coli. Overexpression of wild-type TraQ in the partially resistant strain of E. coli caused both TraA protein content, including propilin and pilin, and Qb amplification to recover to levels comparable with observed in the susceptible strain. These results suggest the possibility that the mutation in TraQ may cause heterogeneity within the E. coli population, with a small number of cells with F pili supporting the phage population and a large number of cells without F pili supporting the E. coli population.

Research Products

• [Journal Article] (2012)
  • Author(s): Tomonori Inomata, Hitomi Kimura, Haruki Hayasaka, Akinori Shiozaki, Yasuhiro Fujita, and Akiko Kashiwagi
  • Journal Title: Archives of Virology Volume: Vol.157(11) Pages: 2163-2169
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Akiko Kashiwagi and Tetsuya Yomo
  • Journal Title: PLoS Genetics Volume: Vol.7 (8) Pages: e1002188
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Saburo Tsuru, Nao Yasuda, Yoshie Murakami, Junya Ushioda, Kotaro Mori, Akiko Kashiwagi, Shingo Suzuki, Bei-Wen Ying, and Tetsuya Yomo
  • Journal Title: Molecular Systems Biology Volume: Vol.7 Pages: 493
  • Peer Reviewed
• [Presentation] (2013)
  • Author(s): 柏木明子,菅原竜,熊谷知史,對馬(佐野)文恵,熊坂直也,四方哲也
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島国際会議場(広島県)
  • Year and Date: 20130918-20
• [Presentation] (2013)
  • Author(s): 柏木明子,菅原竜,對馬(佐野)文恵,熊谷知史,四方哲也
  • Organizer: 日本進化学会第15回筑波大会
  • Place of Presentation: 筑波大学(茨城県)
  • Year and Date: 20130828-31
• [Presentation] (2012)
  • Author(s): 菅原竜,對馬(佐野)文恵,熊谷知史,四方哲也,柏木明子
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡(福岡県)
  • Year and Date: 20121211-14
• [Presentation] (2012)
  • Author(s): Akiko Kashiwagi and Tetsuya Yomo
  • Organizer: 日本進化学会第14回東京大会
  • Place of Presentation: 首都大学東京(東京都)
  • Year and Date: 20120821-24
• [Presentation] (2011)
  • Author(s): 菅原竜,四方哲也,柏木明子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 20111213-16
• [Presentation] (2011)
  • Author(s): 柏木明子,四方哲也
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 20110920-23
• [Presentation] (2011)
  • Author(s): Akiko Kashiwagi and Tetsuya Yomo
  • Organizer: International Union of Microbiological Societies 2011 Congress (IUMS 2011)
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 20110906-10
• [Presentation] (2011)
  • Author(s): 柏木明子,四方哲也
  • Organizer: 日本進化学会第13回大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 20110729-31
• [Presentation] (2011)
  • Author(s): Akiko Kashiwagi and Tetsuya Yomo
  • Organizer: SMBE (Annual congerence of society for molecular biology and evolution)
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 20110726-30
• [Remarks]
  • URL: http://nature.cc.hirosaki-u.ac.jp/public/akiko-kashiwagi/