• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2013 Fiscal Year Final Research Report

Establishment of the haploid breeding method using kinetochore malfunction lines

Research Project

  • PDF
Project/Area Number 23580007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Breeding science
Research InstitutionOkayama University

Principal Investigator

NAGAKI Kiyotaka  岡山大学, 資源植物科学研究所, 准教授 (70305481)

Project Period (FY) 2011 – 2013
Keywords半数体 / 動原体
Research Abstract

In this study, I tried to establish the haploid breeding method using kinetochore malfunction lines. First, plants expressing GFP::CENH3 (centromere specific histone H3) were made by transformations using Arabidopsis, tobacco and tomato for the purpose. Next, RNAi constructs to knock down internal CENH3 were transformed to the plants. Some transformants having these constructs were isolated but expression levels of internal CENH3 in the transformants were as same as these in wild type. These results suggested that RNAi method is not sufficient for the purpose.

URL: 

Published: 2015-07-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi