Maintenance of membrane integrity by phospholipid remodeling in yeast

2014 Fiscal Year Final Research Report

• Project/Area Number: 23580104
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: FUKUDA Ryouichi 東京大学, 農学生命科学研究科, 助教 (50323481)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 酵母 / ホスファチジルコリン / ホスファチジルエタノールアミン / リモデリング

Outline of Final Research Achievements

The remodeling of phospholipids contributes to the generation of the diversity of phospholipid species and to the maintenance of membrane homeostasis. In this study, the molecular mechanisms of the remodeling of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in the yeast Saccharomyces cerevisiae were analyzed. We constructed a system to evaluate the acyl chain remodeling of PC using a yeast mutant defective in PC synthesis and PC containing short acyl chains. In addition, systems to specifically analyze the introduction of acyl residues at sn-1 positions of PC and PE using newly synthesized analogs in vivo and in vitro were constructed. Acyltransferases that introduce acyl residues at sn-1 positions of these analogs were identified.

Free Research Field

微生物学