2013 Fiscal Year Final Research Report
Search on new function of cell-cycle-inhibitors in higher plant.
| Project/Area Number |
23580127
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 細胞周期 / 細胞周期停止因子 / kip-related protein / シロイヌナズナ / 高等植物 |
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| Research Abstract |
To search on new function of cell-cycle-inhibitor in higher plant,I focused Arabidopsis KRPs (Kip-related proteins).I isolated many candidates for AtKRP-binding proteins by Yeast two hybrid system before the application. In this study, I checked in vitro and in vivo interaction between AtKRP and the candidates. At least, one of candidate could sedimente with AtKRP by using pull-down assay.The candidate applied to define the binding area with AtKRP by usig yeast-two-hybrid assay system, and the area was defined. Another candidate(s) might be bound with AtKRP in the Arabidopsis culture T87-cells. I also prepared overexpressing-mutant for AtKRP in Arabidopsis plant. Two homo-lines were established for the mutants.
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