2013 Fiscal Year Final Research Report
Carbohydrate-recognition and transmembrane ion-channel-formation mechanisms of calcium-dependent lectins
| Project/Area Number |
23580137
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UNNO Hideaki 長崎大学, 大学院工学研究科, 助教 (10452872)
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| Project Period (FY) |
2011 – 2013
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| Keywords | レクチン / 糖鎖 / 部位特異的変異 / X線結晶構造解析 / 蛋白質工学 |
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| Research Abstract |
Carbohydrate-recognition mechanisms of calcium-dependent lectins, which play important roles in molecular recognition systems in organisms, were analyzed, and a novel artificial lectin with a designed binding-specificity was produced. In addition, site-directed mutational and three-dimensional structural analyses of the hemolytic lectin CEL-III revealed that it forms heptamers in target cell membrane with extensive conformational changes, forming the transmembrane pores that lead to hemolysis or cell death. These results may provide valuable information on various pore-forming toxin proteins widely distributed in the nature.
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