2013 Fiscal Year Final Research Report
The analysis of auxin biosynthetic pathway using metabolic intermediates
| Project/Area Number |
23580144
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
SUZUKI Masashi 横浜市立大学, 付置研究所, その他 (30342801)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIMADA Yukihisa 横浜市立大学, 木原生物学研究所, 教授 (30300875)
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| Project Period (FY) |
2011 – 2013
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| Keywords | インドールピルビン酸 / オーキシン / 生合成 / 阻害剤 / フィードバック / YUCCA |
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| Research Abstract |
In this research, a feedback regulatory mechanism of auxin biosynthesis has been studied by transcription analysis of auxin biosynthetic genes and metabolic analysis in auxin excess and deficient condition of Arabidopsis seedlings. Auxin excess and deficient conditions were produced by feeding of synthetic auxin and auxin biosynthesis inhibitors to wild type Arabidopsis and by using genetic mutants in auxin biosynthesis. As a result, auxin biosynthesis has been demonstrated to be regulated at the step of YUCCA, a rate-limiting step of auxin biosynthesis, through the negative feedback manner. This suggests that auxin biosynthesis as well as conjugation and degradation contributes to auxin homeostasis. We consider that our results may be useful for agricultural technology such as promotion of rooting and stable fruiting.
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[Journal Article] Analysis of a putative auxin biosynthesis inhibitor, indole-3-oxoethylphosphonic acid, in Arabidopsis2014
Author(s)
Ishida Y, Hayashi K, Soeno K, Asami T, Nakamura S, Suzuki M, Nakamura A, Shimada Y.
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Journal Title
Bioscience, Biochemistry and Biotechnology
Volume: 78 巻
Pages: 67-70
DOI
Peer Reviewed
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[Presentation] Characterization of a Dof-type transcription factor, which plays a negative role in auxin signaling in Arabidopsis root elongation2012
Author(s)
Ishida Y, Nakamura A, Suzuki M, Kakei Y, Kondou Y, Matsui M, Toyooka K, Hayashi K, Asami T, Shimada Y.
Organizer
AUXIN2012
Place of Presentation
Hawaii, USA
Year and Date
20121209-14
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