2013 Fiscal Year Final Research Report
Evaluation of cell-penetrating peptides for understanding the plant immune system and its application to a screening method for novel plant defense activators
| Project/Area Number |
23580148
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 植物 / 免疫機構 / 細胞透過 / ペプチド / plant activator |
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| Research Abstract |
Cell-penetrating peptides (CPPs) can cross the cellular membrane, which is used to carry a functional peptide or protein inside the cell. Oligo-arginine peptides, which consist of 4-12 arginine residues, are one of well-studied CPPs. Oligo-arginine is also able to penetrate into plant cells, but significant adsorption of the peptide to the cell wall was observed, which might hamper the effective penetration. In this study, to obtain more effective CPPs against plant cells we evaluated the cell penetration of pVEC, which is known to penetrate cells in a different fashion to oligo-arginine. As a consequence, pVEC showed approximately 4-times higher penetration into tobacco cells than oligo-arginine. In addition, relatively low adsorption of pVEC to the cell wall was observed, which depends on the N-terminal hydrophobic region. These results indicate that pVEC is a useful tool for understanding the intracellular recognition of pathogen infection.
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