2013 Fiscal Year Final Research Report
Elucidation of strigol biosynthesis in Menispermum dauricum
| Project/Area Number |
23580149
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
MIZUTANI Masaharu 神戸大学, (連合)農学研究科(研究院), 准教授 (60303898)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SUGIMOTO Yukihiro 神戸大学, 大学院農学研究科, 教授 (10243411)
UENO Kotomi 神戸大学, 大学院農学研究科, 術推進研 究員 (40582028)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | ストリゴラクトン / コウモリカズラ / ストリゴール / GR24 / ソルガム / ソルゴモール / P450 |
|---|
| Research Abstract |
Deuterium-labeled 5-deoxystrigol was synthesized, and the analytical method for strigolactones by UPLC-ESI-MS was established. When the root-cultures of Menispermum dauricum, which are a high producer of strigol, were fed with deuterium-labeled 5-deoxystrigol, the conversion of 5-deoxystrigol to strigol was not detected. This suggests that strigol is not biosynthesized via 5-deoxystrigol in Menispermum dauricum. On the other hand, GR24, a synthetic strigolactone analog, was metabolized to 7-OH-GR24 by the root cultures. Next, deuterium-labeled 5-deoxystrigol was administered to hydroponics of a high sorgomol-producing sorghum cultivar, Sorghum bicolor (L.) Moench, and conversion to sorgomol was investigated. 5-deoxystrigol was metabolized to sorgomol and the conversion was inhibited by a P450 inhibitor. These results suggest that sorgomol is biosynthesized from 5-deoxystrigol via a P450-dependent C-9 hydroxylation.
|
Research Products
(18 results)
