2013 Fiscal Year Final Research Report
Cellulose hydorogel for three-dimensional scaffold of cell culture
| Project/Area Number |
23580226
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
WADA Masahisa 東京大学, 農学生命科学研究科, 准教授 (40270897)
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Satoshi 東京大学, 大学院農学生命科学研究科, 助 教 (00420224)
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| Project Period (FY) |
2011 – 2013
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| Keywords | セルロース / ヒドロゲル / 孔径制御 / 細胞培養 / 足場材料 |
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| Research Abstract |
Cellulose was completely dissolved in the 60 wt% lithium bromide aqueous solution in the temperature 120 degrees. Then the cellulose solution was poured into glass container containing large excess amount of salt (NaCl) particles that was heated at 120 degrees in advance. The cellulose was regenerated from the solution by cooling down to room temperature and removing the slats by washing with water. The cellulose gel thus obtained has a uniform and large pore size more than 0.1 mm in diameter. The particle size of salts used in the process leads to the control of morphology and mechanical properties of the gel. The gels were seeded with rabbit chondrocyte and cultured in vitro for three weeks, and proliferation of chondrocyte and its differentiation to cartilagines were observed. In consequence, the cellulose gel has a possibility using as a three-dimensional scaffold for cell culture of tissue engineering.
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