2013 Fiscal Year Final Research Report
Taxonomic determination of kuchijirosho virus by genomic analysis.
| Project/Area Number |
23580261
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Fukui Prefectural University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUETAKE Hiroaki 福井県立大学, 海洋生物資源学部, 准教授 (00334326)
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| Co-Investigator(Renkei-kenkyūsha) |
ISSHIKI Tadashi 三重大学, 大学院生物資源学研究科, 准教 授 (30378319)
KITAMURA Shin-ichi 愛媛大学, 沿岸環境科学研究センター, 准 教授 (40448379)
TAKEUCHI Kenji 福井大学, 医学部, 助教(学内講師) (40236419)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ウイルス / トラフグ / 口白症 / 魚病 / ゲノム解析 |
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| Research Abstract |
Kuchijirosho virus infects cultured fugu (Takifugu rubripes) and occured epizootic outbreaks in aquaculture farm in Japan. Physicochemical characterization and electron microscopic observation suggested that kuchijiroshou virus can belong to Genus Flavivirus, or at least Flaviviridae.
We tried to RT-PCR amplification using 20 sets of PCR primer which are universal to Flavivirus, Hepacivirus, or Pestivirus; however, any virus-specific nucleotide sequence was amplified.
"Rapid Determination System for Viral RNA Sequences" method was adopted to determine unknown virus genome nucleotide sequence. We succeeded first to clone 3 cDNA fragments from brains of kuchijirosho-infected fugu. Although These RNAs appeared in only kuchijirosho-affected brain, it is not clear that these RNAs are derived from kuchijirosho genome itself. So, we call these RNAs "kuchijirosho-assochiated RNA, KAR". To determine nucleotide sequence of whole genome, we are now perfoming next generation sequencer.
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