2013 Fiscal Year Final Research Report
The role of vector saliva in transmission of vector-borne diseases
| Project/Area Number |
23580424
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
KATO HIROTOMO 北海道大学, (連合)獣医学研究科, 准教授 (00346579)
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| Project Period (FY) |
2011 – 2013
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| Keywords | サシチョウバエ / 唾液 / リーシュマニア / 生理活性物質 |
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| Research Abstract |
The salivary gland transcriptome analysis of Lutzomyia ayacuchensis, a vector of cutaneous leishmaniasis in Ecuadorian Andes, was performed and abundant transcripts coding for secreted proteins were identified. The most abundant transcript coded for an RGD-containing peptide was expressed in Escherichia coli and its activity was characterized. The resulting protein inhibited both platelet aggregation and blood coagulation pathway, indicating that the peptide plays an important role in the blood feeding of Lu. ayacuchensis by inhibiting host hemostasis via dual mechanisms. On the other hand, salivary nucleotidases of sand flies were assessed on their activity as an exacerbation factor for Leishmania infection. Salivary nucleotidases, PduApy and PduADA, slightly accelerated the disease progression after co-injection of Leishmania parasites, suggesting that these proteins in saliva may be partly associated with exacerbation of the disease in leishmaniasis.
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Research Products
(46 results)
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[Journal Article] The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina2014
Author(s)
Locatelli FM, Cajal SP, Barroso PA, Lauthier JJ, Mora MC, Juarez M, Kato H, Nasser JR, Hashiguchi Y, Korenaga M, Marco JD.
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Journal Title
Acta Trop
Volume: 131
Pages: 16-21
Peer Reviewed
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[Journal Article] Analysis of salivary gland transcripts of the sand fly Lutzomyia ayacuchensis, a vector of Andean-type cutaneous leishmaniasis2013
Author(s)
Kato H, Jochim RC, Gomez EA, Uezato H, Mimori T, Korenaga M, Sakurai T, Katakura K, Valenzuela JG, Hashiguchi Y.
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Journal Title
Infect Genet Evol
Volume: 13
Pages: 56-66
Peer Reviewed
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[Journal Article] Genetic diversity of the mitochondrial cytochrome b gene in Lutzomyia spp., with special reference to Lutzomyia peruensis, a main vector of Leishmania (Viannia) peruviana in the Peruvian Andes2013
Author(s)
Yamamoto K, Cáceres AG, Gomez EA, Mimori T, Iwata H, Korenaga M, Sakurai T, Katakura K, Hashiguchi Y, Kato H.
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Journal Title
Acta Trop
Volume: 126
Pages: 156-163
Peer Reviewed
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[Journal Article] Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic2012
Author(s)
Fujita M, Kato H, Cáceres AG, Gomez EA, Velez L, Mimori T, Zhang F, Iwata H, Korenaga M, Sakurai T, Katakura K, Hashiguchi Y.
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Journal Title
Acta Trop
Volume: 121
Pages: 93-98
Peer Reviewed
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[Journal Article] Dimiconin, a novel coagulation inhibitor from the kissing bug, Triatoma dimidiata, a vector of Chagas disease2012
Author(s)
Ishimaru Y, Gomez EA, Zhang F, Martini-Robles L, Iwata H, Sakurai T, Katakura K, Hashiguchi Y, Kato H.
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Journal Title
J Exp Biol
Volume: 215
Pages: 3597-3602
Peer Reviewed
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