2013 Fiscal Year Final Research Report

Trial of new virus isolation from industrial animals

Research Project

Project/Area Number	23580451
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Clinical veterinary science
Research Institution	Azabu University
Principal Investigator	OCHIAI Hideharu 麻布大学, 付置研究所, 准教授 (20247307)
Co-Investigator(Kenkyū-buntansha)	MIZUTANI Tetsuya 国立感染症研究所, ウイルスII部 (70281681)
Project Period (FY)	2011 – 2013
Keywords	ウイルス / 衛生 / 感染症
Research Abstract	Goat diarrheal feces were subjected to metagenome analysis by the next-generation sequencing. Nucleotide sequences with homology to enteroviruses were obtained. Primers for RT-PCR were designed based on the nucleotide sequence of these sequences at the 5'-untranslated region, and we determined 563 bp nucleotide sequences that showed homology to bovine-like and ovine enteroviruses (77–87 %). We named the virus detected in this study goat enterovirus G1 (GEV-G1). In the phylogenetic analysis, GEV-G1 belonged to a cluster containing ovine enteroviruses. To our knowledge, this is the first report on nucleotide sequences of an enterovirus infecting Japanese goats.

(1 results)