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2013 Fiscal Year Final Research Report

Development of microbial factory for bioplastic production adapting inedible biomass hydrolysates

Research Project

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Project/Area Number 23580452
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Boundary agriculture
Research InstitutionHokkaido University

Principal Investigator

OOI Toshihiko  北海道大学, 工学(系)研究科(研究院), 准教授 (40223713)

Co-Investigator(Kenkyū-buntansha) TAGUCHI Seiichi  北海道大学, 大学院工学研究院, 教授 (70216828)
KEN'ICHRO Matsumoto  北海道大学, 大学院工学研究院, 准教授 (80360642)
Project Period (FY) 2011 – 2013
Keywordsバイオプラスチック / バイオポリエステル / 非可食バイオマス / 微生物工場 / 糖化 / 乳酸ポリマー
Research Abstract

Lignocellulosic biomass is mainly composed of glucose and xylose as the major sugars. The use of glucose for the biopolymer production has been demonstrated to be efficient whereas, the use of xylose is inefficient. However efficient utilization of both sugars is essential. In this study, methods for the efficient utilization of xylose were developed and enhanced to obtain high productivities of biopolymers. For P(LA-co-3HB) production lactic acid supplementation into the cell contributed to the inprovement of polymer yield from xylose. In addition xylose was found to be superiorb to glucose in giving higher LA fraction in the copolymer. The uptake of xylose by GatC which is galactitol transporter also effective to produce the copolymer. The approach for high yield production of biopolymers in combination with the lignocellulosic-derived mix sugars have a potential for the establishment of biorifinary for the production of variety of biopolymers.

  • Research Products

    (13 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (9 results) Remarks (1 results)

  • [Journal Article] Enhanced production of poly(lactate-co-3- hydroxybutyrate) from xylose in engineered Escherichia coli overexpressing a galactitol transporter2014

    • Author(s)
      JM. Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Journal Title

      Appl. Microbiol. Biotechnol

      Volume: vol.98, No. 6 Pages: 2453-2460

    • DOI

      10.1007/s00253-013-5401-0

    • Peer Reviewed
  • [Journal Article] Effectiveness of xylose utilization for high yield production of lactate-enriched P(lactate-co-3- hydroxybutyrate) using a lactate-overproducing strain of Escherichia coli and an evolved lactate-polymerizing enzyme2013

    • Author(s)
      JM. Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Journal Title

      Metab. Eng

      Volume: vol.15 Pages: 159-166

    • DOI

      10.1016/j.ymben.2012.11.007

    • Peer Reviewed
  • [Journal Article] Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance against 5-hydroxymethylfulfural2012

    • Author(s)
      JM. Nduko, W. Suzuki, K. Matsumoto, H. Kobayashi, T. Ooi, A. Fukuoka, S. Taguchi
    • Journal Title

      J. Biosci. Bioeng

      Volume: vol.113, No. 1 Pages: 70-72

    • DOI

      10.1016/j.jbiosc.2011.08.021

    • Peer Reviewed
  • [Presentation] 植物バイオマス由来の酵素糖化物を炭素源としたバイオポリエステルの生産2014

    • Author(s)
      大井俊彦、佐々木勝平、松本謙一郎、田口精一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      明治大学(川崎
    • Year and Date
      20140327-30
  • [Presentation] Efficient production of poly(lactate-co-3- hydroxybutyrate) using hemicellulose-derived sugar, xylose, in engineered Escherichia coli overexpressing a galactitol transporter2013

    • Author(s)
      JM. Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Organizer
      Frontier Chemistry Center International Symposium 2013
    • Place of Presentation
      Hokkaido University (Sapporo)
    • Year and Date
      20131209-10
  • [Presentation] 植物バイオマスを用いた共重合ポリエステルの生産2013

    • Author(s)
      木勝平、大井俊彦、ンドゥコジョン、松本謙一郎、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島)
    • Year and Date
      20130918-20
  • [Presentation] 非可食性植物バイオマスからのP(3HB)の微生物生産2013

    • Author(s)
      大井俊彦、佐々木勝平、ンドゥコジョン、松本謙一郎、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島)
    • Year and Date
      20130918-20
  • [Presentation] Efficient bioconversion of lignocellulosic biomass-derived sugars into poly(lactate-co-3- hydroxybutyrate) by metabolically engineered Escherichia coli2012

    • Author(s)
      JM. Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Organizer
      15th International Biotechnology Symposium and Exhibition
    • Place of Presentation
      EXICO, Daegu (Korea)
    • Year and Date
      20120916-21
  • [Presentation] Lactate-based polyester production by recombinant bacteria using lignocellulosic biomass sugars as carbon sources2012

    • Author(s)
      JM. Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Organizer
      Japan-China-Korea Joint Symposium on Enzyme Engineering
    • Place of Presentation
      Kanazawa Excel Hotel (Kanazawa)
    • Year and Date
      20120528-31
  • [Presentation] Biosynthesis of polyhydroxyalkanoates from cellulose hydrolysate in Escherichia coli strain with superior tolerance to 5-hydroxymethylfurfural2012

    • Author(s)
      JM Nduko, K. Matsumoto, H. Kobayashi, T. Ooi, A. Fukuoka, S. Taguchi
    • Organizer
      The 5th GCOE International Symposium
    • Place of Presentation
      Hokkaido University (Sapporo)
    • Year and Date
      2012-02-22
  • [Presentation] Production of lactate-based polyesters from xylose in Escherichia coli2011

    • Author(s)
      JM Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京農工大学(東京)
    • Year and Date
      20110926-28
  • [Presentation] Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance against 5-hydroxymethylfulfural2011

    • Author(s)
      JM Nduko, W. Suzuki, K. Matsumoto, H. Kobayashi, T. Ooi, A. Fukuoka, S. Taguchi
    • Organizer
      2011 Taiwan-Japan Bilateral Polymer Symposium
    • Place of Presentation
      National China University (Taiwan)
    • Year and Date
      2011-09-15
  • [Remarks]

    • URL

      http://www.eng.hokudai.ac.jp/labo/seika/TOP.html

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Published: 2015-07-16  

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