2013 Fiscal Year Final Research Report
Research on the development of highly improved method for bioleaching from low-grade sulfide ores
| Project/Area Number |
23580458
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Okayama University |
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Principal Investigator |
KAMIMURA Kazuo 岡山大学, その他の研究科, 教授 (80294445)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 鉄酸化細菌 / 硫黄酸化 / チオ硫酸デヒドロゲナーゼ / バクテリアリーチング / 応用微生物学 / 遺伝子発現制御 |
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| Research Abstract |
An iron-oxidizing bacterium, Acidithiobacillus ferrooxidans, is one of the key organisms used in industrial bioleaching applications. Both iron- and sulfur-oxidizing activities are necessary for bioleaching. However, expressions of genes involved in sulfur oxidation are strongly repressed in the presence of ferrous iron. In this research, a protein (ScpB) thought to be involved in the transcriptional regulation of suifide:quinone reductase gene was examined. The transcription of scpB gene was highly activated in sulfur-grown cells. ScpB could bind to putative promoter regions of genes involved in iron or RISC oxidation. Although thiosulfate metabolism in this bacterium has not been characterized, a new gene involved in thiosulfate metabolism has been identified. The properties of the enzyme (thiosulfate dehydrogenase) was characterized.
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