2013 Fiscal Year Final Research Report
The study on molecular functions of the novel RNAi factors DRH-3 and E1 which are required to maintain the chromosome integrity in higher eukaryote
| Project/Area Number |
23590074
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Toyohashi University of Technology |
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Principal Investigator |
EKI Toshihiko 豊橋技術科学大学, 工学(系)研究科(研究院), 教授 (40192512)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ダイサー関連ヘリカーゼ / RNA干渉 / 線虫 / タンパク質機能解析 |
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| Research Abstract |
This study has aimed to clarify the molecular function of two RNAi factors DRH-3 (Dicer Related Helicase-3) and E1 (a novel protein with two Tudor domains) that I originally found to be indispensable for germ-line formation in C. elegans. In this study, I generated the DNA constructs for expression of 10 mutated proteins and successfully prepared His- and GST-tagged-native and some of mutated versions of DRH-3 and E1 proteins using the E. coli protein expression system, and showed the physical interaction between DRH-3 and E1 by pull-down method with differently tagged-native proteins. In addition, genetic and cytological observations on both genes have suggested that DRH-3 and E1 proteins act in the same pathway of RNAi in nucleus.
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