Intracellular transport of phospholipids

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590080
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Kyushu University
• Principal Investigator: KUGE Osamu 九州大学, 理学(系)研究科(研究院), 教授 (30177977)
• Project Period (FY): 2011 – 2013
• Keywords: 生体膜 / リン脂質

Research Abstract

Intracellular transport of phospholipids is an essential event for the biogenesis of functional biological membranes. However, the transport processes are largely unknown with respect to mechanisms, genes and gene products involved. In this study I tried to identify novel genes involved in the phospholipids transport or phospholipid metabolism in yeast, and identified two genes that probably functioned in phospholipid transport to mitochondrial inner membrane, and one gene that was involved in the expression of a phospholipid metabolism gene, PSD2.

Research Products

• [Journal Article] Involvement of Sac1 phosphoinositide phosphatase in the metabolism of phosphatidylserine in the yeast Saccharomyces cerevisiae (2014)
  • Author(s): Tani M, Kuge O
  • Journal Title: Yeast Volume: 31(4) Pages: 145-58
  • DOI: 10.1002/yea.3004
  • Peer Reviewed
• [Journal Article] ミトコンドリアにおけるリン脂質の生合成医学のあゆみ (2014)
  • Author(s): 久下理
  • Volume: 248
  • Pages: 1159-65
  • URL: http://www.ishiyaku.co.jp/magazines/ayumi
• [Journal Article] Tam41 is a CDP-diacylglycerol synthase required for cardiolipin biosynthesis in mitochondria (2013)
  • Author(s): Tamura Y, Harada Y, Nishikawa S, Yamano K, Kamiya M, Shiota T, Kuroda T, Kuge O, Sesaki H, Imai K, Tomii K, Endo T
  • Journal Title: Cell Metab Volume: 17(5) Pages: 709-18
  • DOI: 10.1016/j.cmet.2013.03.018
  • Peer Reviewed
• [Journal Article] Involvement of complex sphingolipids and phosphatidylserine in endosomal trafficking in yeast Saccharomyces cerevisiae (2012)
  • Author(s): Tani M, Kuge O
  • Journal Title: Mol Microbiol Volume: 86(5) Pages: 1262-80
  • DOI: 10.1111/mmi.12057
  • Peer Reviewed
• [Journal Article] Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis (2012)
  • Author(s): Lee S, Uchida Y, Emoto K, Umeda M, Kuge O, Taguchi T, Arai H
  • Journal Title: Genes Cells Volume: 17(8) Pages: 728-36
  • DOI: 10.1111/j.1365-2443.2012.01622.x
  • Peer Reviewed
• [Journal Article] Hydroxylation state of fatty acid and long-chain base moieties of sphingolipid determine the sensitivity to growth inhibition due to AUR1 repression in Saccharomyces cerevisiae (2012)
  • Author(s): Tani M, Kuge O
  • Journal Title: Biochem Biophys Res Commun Volume: 417(2) Pages: 673-8
  • DOI: 10.1016/j.bbrc.2011.11.138
  • Peer Reviewed
• [Presentation] 酵母ホスホイノシチドホスファターゼSAC1欠損によるホスファチジルセリン量低下のメカニズム解析 (2013)
  • Author(s): 谷元洋, 久下理
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2013-09-11
• [Presentation] 複合スフィンゴ脂質とホスファチジルセリンによるエンドソームリサイクリング経路の制御機構 (2012)
  • Author(s): 谷元洋, 久下理
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-15
• [Presentation] 出芽酵母の生育阻害誘導に必要なセラミドの構造的要因の解析 (2012)
  • Author(s): 谷元洋, 久下理
  • Organizer: 日本脂質生化学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-06-08
• [Presentation] 酵母ホスファチジルセリン脱炭酸酵素2の活性発現に関与する新規遺伝子の同定と解析 (2012)
  • Author(s): 久下理、三好琢弥、山口剛典、谷元洋
  • Organizer: 日本脂質生化学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-06-07
• [Presentation] ミトコンドリア新規形態制御因子IAT1の解析 (2012)
  • Author(s): 友廣志穂、大橋順、前田敏孝、山本祐華、谷元洋、久下理
  • Organizer: 日本生化学会九州支部例会
  • Place of Presentation: 福岡
  • Year and Date: 2012-05-27
• [Presentation] 出芽酵母のエンドソーム・ゴルジ間輸送における複合スフインゴ脂質とホスファチジルセリンの機能 (2011)
  • Author(s): 谷元洋, 久下理
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] 酵母ホスファチジルセリン脱炭酸酵素2の活性に関与する新規遺伝子(GIP3)の同定と解析 (2011)
  • Author(s): 三好琢弥、中嶋友博、谷元洋、久下理
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22