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2013 Fiscal Year Final Research Report

Functional analysis of separase and its novel substrate in the licensing of centrosome duplication

Research Project

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Project/Area Number 23590087
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionTeikyo Heisei University

Principal Investigator

TAKAHASHI Mikiko  帝京平成大学, 薬学部, 教授 (90324938)

Co-Investigator(Kenkyū-buntansha) TADA Shusuke  帝京平成大学, 薬学部, 教授 (00216970)
MUKAI Hideyuki  神戸大学, バイオシグナル研究センター, 准教授 (80252758)
MATSUO Kazuhiko  帝京平成大学, 薬学部, 助教 (70599753)
Project Period (FY) 2011 – 2013
Keywords中心体 / 細胞周期 / 中心体複製 / 分裂期 / セパレース
Research Abstract

Centrosome assembles the bipolar spindle during mitosis to ensure the equal segregation of replicated chromosomes. It is thereby assumed that the licensing mechanism tightly regulates the initiation of centrosome duplication only once per cell cycle, though precise process remains largely unknown. Separase, a cysteine protease that triggers sister chromatid separation, is known to be involved in this licensing, however, its centrosomal substrate remains unidentified. In this study, we found that the centrosomal protein kendrin is cleaved by separase at a consensus site. Moreover, expression of a non-cleavable kendrin mutant suppresses the initiation of centrosome duplication. Our finding provides the first evidence that kendrin is a novel and crucial substrate for separase at the centrosome involved in the licensing of centrosome duplication.

  • Research Products

    (6 results)

All 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] More isn't always better : Limiting centrosome size in interphase2013

    • Author(s)
      Mikiko Takahashi and Kazuhiko Matsuo
    • Journal Title

      Cell Cycle

      Volume: 12 Pages: 1482

    • DOI

      10.4161/cc.23879

  • [Journal Article] Kendrin is a novel substrate for separase involved in the licensing of centriole duplication2012

    • Author(s)
      Kazuhiko Matsuo, Keita Ohsumi, Mari Iwabuchi, Toshio Kawamata, Yoshitaka Ono, and Mikiko Takahashi
    • Journal Title

      Curr. Biol

      Volume: 22 Pages: 915-921

    • DOI

      10.1016/j.cub.2012.03.048

    • Peer Reviewed
  • [Presentation] Functional analysis of centrosomal protein kendrin in centriole duplication2013

    • Author(s)
      松尾和彦
    • Organizer
      第86回生化学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2013-09-11
  • [Presentation] Possible role of a novel substrate for separase in the licensing of centriole duplication2011

    • Author(s)
      高橋美樹子
    • Organizer
      第34回分子生物学会年会シンポジウム
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-16
  • [Presentation] 中心体複製制御機構におけるseparaseの新規基質候補蛋白質の解析2011

    • Author(s)
      松尾和彦
    • Organizer
      第34回分子生物学会年会シンポジウム
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-16
  • [Remarks]

    • URL

      http://pharm.thu.ac.jp/research/unit/bunshisaibou.html

URL: 

Published: 2015-07-16  

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