2013 Fiscal Year Final Research Report
Modification of organometallic compound cytotoxicity by incorporated metal atoms and molecular mechanisms underlying the toxicity
| Project/Area Number |
23590156
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Toho University (2013)
Hokuriku University (2011-2012) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NOBUKUNI Yoshitaka 広島大学, 原爆放射線医科学研究所, 准教授 (80295641)
YASUIKE Shuji 愛知学院大学, 薬学部, 教授 (10230210)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 細胞毒性 / ビスマス / アンチモン / ハイブリッド分子 / 有機金属化合物 / ジーントラップ法 / 血管内皮細胞 / CHO細胞 |
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| Research Abstract |
We have already found that organobismuth compounds often exhibit cytotoxicity in vascular endothelial cells. The purpose of the present study was to obtain cell clones that are tolerant to the toxic organobismuth compounds (PMTABi, DAPBi, and TDPBi) form a random gene trap insertional mutants library of CHO cells, to investigate candidate genes involved in the tolerance of the cells, and to clarify the molecular targets of the cytotoxicity of the organobismuth compounds. We succeeded to obtain 97 clones of the tolerant cells and identified candidate genes involved in the tolerance of the cells.
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