2013 Fiscal Year Final Research Report
In vitro approaches to evaluate placental drug transport by using differentiating JEG-3 human choriocarcinoma cells
| Project/Area Number |
23590207
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Osaka Ohtani University |
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Principal Investigator |
IKEDA Kenji 大阪大谷大学, 薬学部, 講師 (10434812)
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| Co-Investigator(Renkei-kenkyūsha) |
UTOGUCHI Naoki 帝京大学, 薬学部, 教授 (80276633)
HIROTANI Yoshihiko 大阪大谷大学, 薬学部, 教授 (00351487)
MYOTOKU Michiaki 大阪大谷大学, 薬学部, 准教授 (30469233)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 胎盤 / モデル / 物質透過 / トロホブラスト / BCRP / DJEG / JEG-3 / 妊娠時薬物療法 |
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| Research Abstract |
Human choriocarcinoma cells have been used as models for studying transcellular drug transport through placental trophoblasts. However, these models allow the transport of low-molecular-weight drugs through intercellular gap junctions. This study aimed to investigate the differentiation patterns of JEG-3 choriocarcinoma cells under different culture conditions and establish the appropriate model of in vitro syncytiotrophoblast drug transport. The differentiation pattern of JEG-3 cultured in CS-C resembled the syncytiotrophoblast-like differentiation signal characterizations in vivo. In conclusion, the syncytiotrophoblast-like models of differentiating JEG-3 cells cultured in CS-C might be appropriate for evaluating drug transport across the placental trophoblast.
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