2013 Fiscal Year Final Research Report
The induction mechanisms for the paracellular transport upon stimulation of salivary secretion
| Project/Area Number |
23590271
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SUGIYA Hiroshi 日本大学, 生物資源科学部, 教授 (20050114)
HASHIMOTO Sadamitsu 東京歯科大学, 教養部, 准教授 (10201708)
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| Research Collaborator |
ADRIAN E Hill Cambridge University, Physiological Laboratory, 准教授
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| Project Period (FY) |
2011 – 2013
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| Keywords | 上皮機能 / 傍細胞輸送 / タイト結合 / 血管灌流唾液腺 / 水輸送 / 細胞内信号 / 蛍光物質分泌 / 駆動力 |
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| Research Abstract |
To clarify the mechanism to start the paracellular transport (more than 60% of the whole fluid secretion of the gland is estimated from paracellular route), the isolated vascularly perfused mandibular gland of rat was placed on the stage of the confocal laser-scanning microscope. We observed the passage of the fluorescent dye (~500 Da) as an indicator of paracellular transport. When the fluid secretion was induced by muscarinic or alpha1-adrenergic stimulation, the fluorescence of each intracellular canalliculi responded variously, but the average of canalliculus increased dose-dependently to the plateau within 30s. The present project led an conclusion that we can observe the entry of fluorescent dye from intercellular space to luminal space by pressure-dependent paracellular route by solvent-drag, and that the fluid secretion through pressure-independent route is still open for further studies. The process of opening continued for 30 s, which was similar to that of cell shrinkage.
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[Remarks] 村上政隆(2012.10.25)生理研共同研究とリポジトリ。大学共同利用機関におけるリポジトリに関する情報交換会。民族学博物館(吹田)
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[Remarks] 村上政隆(2012.10.5)生理学研究所におけるアーカイブズの現況と今後の構想。平成24年度第2回自然科学系アーカイブズ会合。高エネルギー加速器研究機構(つくば)
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[Remarks] 村上政隆(2012.7.17)生理学研究所におけるアーカイブズの現況と今後の構想。平成24年度第1回自然科学系アーカイブズ会合。核融合研究所アーカイブ室(土岐)
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[Remarks] Murakami M (2013.3.10) IADR Distinguished Scientist for Salivary Research 2013 (IADR general session, Seattle, USA)
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