2013 Fiscal Year Final Research Report
The pharmacological properties of tonic currents mediated by GABAA-Rs in BLA neurons
| Project/Area Number |
23590276
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Hirosaki University |
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Principal Investigator |
YAMADA JUNKO 弘前大学, 医学(系)研究科(研究院), 講師 (30334965)
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| Co-Investigator(Renkei-kenkyūsha) |
UENO Shinya 弘前大学, 大学院医学研究科, 教授 (00312158)
MIGITA Keisuke 弘前大学, 大学院医学研究科, 助教 (10352262)
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| Project Period (FY) |
2011 – 2013
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| Keywords | GABA / 抑制 / トニック抑制 / 不安 / 性差 |
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| Research Abstract |
Synaptically released GABA acting on postsynaptic GABAA receptor produce not only phasic inhibition, but also tonic inhibition by persistent activation of extrasynaptic receptors. This study aimed at examining pharmacological properties of tonic currents mediated by GABAA-Rs in BLA neurons of sex differences using the wild type and PRIP-1 KO mice which is known of downregulation of GABAergic transmission. The application of GABAA-R antagonist bicuculline blocked tonic GABAergic current which was significantly larger than those in KO. Bath application of diazepam and zolpidem causes increase tonic conductance in WT. In contrast, PRIP-1 KO mice showed lower sensitivity to these compounds. These data indicate that tonic GABAergic inhibition exist in principal neurons of the basolateral amygdala in WT mice but PRIP-1 KO mice have imperfection of this inhibitory system.
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Research Products
(14 results)
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[Journal Article] Morphologic changes of dendritic spines of striatal projection neurons in a rat model of L-DOPA-induced dyskinesia2014
Author(s)
Nishijima H, Suzuki S, Kon T, Funamizu Y, Ueno T, Haga R, Miki Y, Suzuki C, Arai A, Kimura T, Suzuki C, Meguro R, Yamada J, Migita K, Ichinohe N, Ueno S, Baba M, Tomiyama M
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Journal Title
Movement Disorders
Volume: 29(3)
Pages: 336-343
DOI
Peer Reviewed
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[Journal Article] A novel prophylactic effect of furosemide treatment on autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE)2013
Author(s)
Yamada J., Zhu G., Okada M., Hirose S., Yoshida S., Shiba Y., Migita K., Mori F., Sugawara T., Chen L., LiuF., Yoshida S., Ueno S., Kaneko S
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Journal Title
Epi. Res
Volume: 107(1-2)
Pages: 127-137
DOI
Peer Reviewed
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[Journal Article] Taurine Inhibits K+-Cl- Cotransporter KCC2 to Regulate Embryonic Cl- Homeostasis via With-no-lysine (WNK) Protein Kinase Signaling Pathway2012
Author(s)
Inoue, K., Furukawa, T., Kumada, T., Yamada J., Wang, TY., Inoue, R., Fukuda
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Journal Title
J. Biol Chem
Volume: 287(25)
Pages: 20839-20850
DOI
Peer Reviewed
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[Journal Article] Dysfunction of extrasynaptic GABAergic transmission in PRIP-1 KO mice is associated with an epilepsy phenotype2012
Author(s)
Zhu G., Yoshida S., Migita K., Yamada J., Mori F., Tomiyama M., Wakabayashi K., Kanematsu T., Hirata M., Kaneko S., Ueno S and Okada M
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Journal Title
J Pharmacol Exp Ther
Volume: 340(3)
Pages: 520-528
DOI
Peer Reviewed
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