2013 Fiscal Year Final Research Report
Effects of restrained water immersion stress on the metabolism of histamine in the gastric mucosa.
| Project/Area Number |
23590316
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Shinya H 兵庫医科大学, 医学部, 講師 (70273703)
NAGANO Takayuki 兵庫医科大学, 医学部, 助教 (10368516)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ヒスタミン / 拘束水浸ストレス / 代謝酵素 |
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| Research Abstract |
Restrained water immersion stress of 30 min (WRS) in mice and rats caused mild ulcer in the gastric mucosa. Histamine content in the gastric mucosa increased 1.6 and 1.3 times, respectively. Anti-histamine N-methyltransferase (HMT) antibody detected a band in rat kidney, but not in gastric mucosa. Quantitative PCR detected HMT mRNA in gastric mucosa, but the expression level did not change by WRS. Anti-histidine decarboxylase antibody detected a single band in the rat gastric mucosa but the content did not change by WRS. Anti-diamine oxidase antibody failed to detect expression in the gastric mucosa. The phosphorylation level of MAP kinases (ERK1/2, SAPK/KNK, p38) did not change by WRS.
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