Identification of acceptor proteins and their modification sites of polyADP-ribosylation and biological function

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590350
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: MIWA Masanao 長浜バイオ大学, バイオサイエンス学部, 教授 (20012750)
• Project Period (FY): 2011 – 2013
• Keywords: 翻訳後修飾 / ポリADP-リボシル化 / ショウジョウバエ変異体 / アクセプタータンパク質

Research Abstract

One of the biological functions of polyADP-ribosylation has been known to be involved in DNA base excision repair. However the other functions are still not known yet. To clarify the functions through identifying the in vivo acceptor proteins of polyADP-ribosylation that is mostly unknown, we used Drosophila melanogaster mutant that had deleted poly(ADP-ribose) degradation enzyme gene, (poly(ADP-ribose) glycohydrolase gene. This mutant Drosophila could eclose under elevated temperature and shows neurological disease. Thus this might serve as a model for human neurological diseases. Using affinity column of immobilized antibody against poly(ADP-ribose), alkaline treatment, 2D gel electrophoresis and mass spectroscopy, we identified alcohol dehydrogenase and several other proteins as candidates of polyADP-ribosylation in vivo.

Research Products

• [Journal Article] Mitochondrial P5, a member of protein disulphide isomerase family, suppresses oxidative stress-induced cell death (2012)
  • Author(s): Shitara, Y., Tonohora, Y., Goto, T., Yamada, Y., Miki, T., Makino, H., Miwa, M., Komiya, T.
  • Journal Title: J Biochem Volume: 152(1) Pages: 73-85
  • DOI: 10.1093/jb/mvs034
  • Peer Reviewed
• [Journal Article] A novel pathway of centrosome amplification that does not require DNA lesions (2012)
  • Author(s): Tsuda, M., Tanaka, M., Mushiake, M., Takahashi, J., Tanaka, K., Watase, J., Fujisawa, J.-I., Miwa, M.
  • Journal Title: Cancer Sci Volume: 103(2) Pages: 191-196
  • DOI: 10.1111/j.1349-7006.2011.02152.x.Epub2011Dec15
  • Peer Reviewed
• [Journal Article] Ink4a and Arf are crucial factors in the determination of the cell of origin and the therapeutic sensitivity of Myc-induced mouse lymphoid tumor (2012)
  • Author(s): Sugihara, E., Shimizu, T., Kojima, K., Onishi, N., Kai, K., Ishizawa, J., Nagata, K., Hashimoto, N., Honda, H., Kanno, M., Miwa, M., Okada, S., Andreeff, M., Saya, H.
  • Journal Title: Oncogene Volume: 31(23)[Epub ahead of print] Pages: 2849-2861
  • DOI: 10.1038/onc.2011.462
  • Peer Reviewed
• [Journal Article] Approaches to separate and identify polyADP-ribosylated proteins using PARG knockout Drosophila (2011)
  • Author(s): Nodono, H., Hamada, A., Kuroda, Y., Kamemura, K., Hasegawa, M., Komiya, T., Kondo, C., Che, F. S., Hanai, S., Miwa, M.
  • Journal Title: Methods Mol Biol Volume: 780 Pages: 377-390
  • DOI: 10.1007/978-1-61779-270-0_22
  • Peer Reviewed
• [Journal Article] PARP proteins, NAD, epigenetics, and antioxidative response in abiotic stress. In : Applied plant genomics and biotechnology
  • Author(s): Poltronieri, P., Miwa, M.
  • Journal Title: Woodhead Publishing, Elsevier, Cambridge, UK(Eds. Palmiro Poltronieri and Yiguo Hong (Invited article)) Volume: (In press)
• [Presentation] Regulation of in vivo level of polyADP-ribosylation, cell growth and centrosome amplification (2014)
  • Author(s): Tanaka, M., Ida, C., Takahashi, J., Eguchi, T., Ohta, N., Yamashita, S., Tsukada, M., Mushiake, M., Fujii, T., Ikegami, S., Nishi, Y., Fujisawa, J., Miwa, M.
  • Organizer: Cold Spring Harbor Laboratory Meeting on The PARP Family & Friends : Gene regulation & beyond
  • Place of Presentation: Cold Spring Harbor Laboratory, New York
  • Year and Date: 20140409-12
• [Presentation] ヒト白血病細胞K562の細胞分化誘導過程における細胞内NAD量およびポリ(ADP-リボース)量の変動 (2013)
  • Author(s): 井田智恵利, 緒方進, 塚田匡輝, 田中正和, 三輪正直
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場(P2-0477)
  • Year and Date: 20131203-06
• [Presentation] 組み替えhuman poly(ADP-ribose) polymerase 1の精製と予備的構造解析 (2013)
  • Author(s): 香山賢一, 江口貴之, 森田寛之, 亀村和生, 西義介, 三輪正直, 白井剛
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20131028-30
• [Presentation] Detection of DNA strand Breaks with ELISA for poly(ADP-ribose) (2013)
  • Author(s): Tsukada, M., Eguchi, T., Yamashita, S., Takahashi, J., Tanaka, M., Fujii, T., Ikegami, S., Miwa, M.
  • Organizer: PARP2013 19^<th> International Conference on ADP-ribosylation
  • Place of Presentation: Centre de Recherche du Centre Hospitalier Universitaire de Quebec-Pavillon CHUL, Quebec city, Canada
  • Year and Date: 20130906-09
• [Presentation] Effect of inhibition of polyADP-ribosylation on cell proliferation in CHO-K1 cells (2013)
  • Author(s): Tanaka, M., Takahashi, J., Eguchi, T., Tsukada, M., Mushiake, M., Fujisawa, J-I., Miwa, M.
  • Organizer: PARP2013 19^<th> International Conference on ADP-ribosylation
  • Place of Presentation: Centre de Recherche du Centre Hospitalier Universitaire de Quebec-Pavillon CHUL, Quebec city, Canada
  • Year and Date: 20130906-09
• [Presentation] ポリADP-リボ-ス分解酵素(PARG)ノックアウトショジョウバエから同定されたalcohol dehydrogenase (ADH)のポリADP-リボシル化の検証 (2012)
  • Author(s): 山下幸子, 江口貴之, 藤井貴弘, 亀村和生, 小宮徹, 蔡晃植, 田中正和, 藤澤順一, 西義介, 三輪正直
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 20121211-14
• [Presentation] 組み替えhuman poly(ADP-ribose) polymerase 1の精製とその立体構造解析 (2012)
  • Author(s): 香山賢一, 江口貴之, 森田寛之, 亀村和生, 西義介, 三輪正直, 白井剛
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 20121211-14
• [Presentation] 生理的条件におけるDNA損傷の程度を測るためのポリ(ADP-リボ-ス)量の測定 (2012)
  • Author(s): 江口貴之, 高橋淳, 貴田亨, 藤井貴弘, 池上晋, 三輪正直
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 20121211-14
• [Presentation] 種々の条件下におけるヒト細胞のポリ(ADP-リボ-ス)量の変化 (2012)
  • Author(s): 太田成美, 江口貴之, 高橋淳, 田中正和, 藤井貴弘, 池上晋, 三輪正直
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 20121211-14
• [Presentation] ELISA法を用いた細胞内ポリ(ADP-リボ-ス)の定量 (2012)
  • Author(s): 江口貴之, 三輪正直, 他
  • Organizer: 第31回分子病理学会(恵那シンポジウム)
  • Place of Presentation: 恵那峡グランドホテル
  • Year and Date: 20120721-22
• [Presentation] ポリ(ADP-リボ-ス)分解酵素(PARG)ノックアウトショウジョウバエに蓄積するタンパク質のポリADP-リボシル化の検証 (2012)
  • Author(s): 山下幸子, 三輪正直, 他
  • Organizer: 第31回分子病理学会(恵那シンポジウム)
  • Place of Presentation: 恵那峡グランドホテル
  • Year and Date: 20120721-22
• [Presentation] Preparation of quantities of recombinant human poly(ADP-ribose) polymerase 1 for structural analysis (2011)
  • Author(s): Koyama, K., Eguchi, T., Morita, H., Sakai, T., Kamemura, K., Nishi, Y., Miwa, M.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(2P-0310)
  • Year and Date: 20111213-16
• [Presentation] ポリADP-リボシル化の阻害と中心体増幅の関係 (2011)
  • Author(s): 寺田佳優, 虫明正敏, 高橋淳, 藤井貴弘, 田中正和, 三輪正直
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(3P-0487)
  • Year and Date: 20111213-16
• [Presentation] Change of the amount of poly(ADP-ribose) in cells under physiological Condition and DNA damage (2011)
  • Author(s): Eguchi, T., Takahashi, J., Kida, T., Fujii, T., Ikegami, S., Miwa, M.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 20111213-16
• [Presentation] Effect of inhibition of polyADP-ribosylation on cell proliferation (2011)
  • Author(s): Takahashi, J., Mushiake, M., Tsuda, M., Tanaka, M., Miwa, M.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(4P-0430)
  • Year and Date: 20111213-16
• [Presentation] Verification of an acceptor protein for polyADP-ribosylation from poly(ADP-ribose) glycohydrolase-knockout Drosophila melanogaster (2011)
  • Author(s): Yamashita, S., Eguchi, T., Nodono, H., Hamada, A., Kuroda, Y., Kamemura, K., Komiya, T., Hanai, S., Miwa, M.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 20111213-16
• [Presentation] Effect of inhibition of polyADP-ribosylation on centrosome and chromosome (2011)
  • Author(s): 三輪正直, 虫明正敏, 高橋淳, 江口貴之, 家村顕自, 大杉美穂, 藤沢順一, 田中正和
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋会議センター
  • Year and Date: 20111003-05
• [Presentation] ポリ(ADP-リボース)分解酵素遺伝子欠失変異による神経細胞死を呈するショウジョウバエのポリADP-リボシル化タンパク質の同定法について (2011)
  • Author(s): 三輪正直, 野殿弘人, 浜田彰子, 黒田泰仁, 亀村和生, 長谷川慎, 小宮徹, 近藤真千子, 藤井貴弘, 蔡晃植, 花井修二
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(シンポジウム1S4a-5)
  • Year and Date: 20110921-24
• [Presentation] Poly(ADP-ribose)polymerase-1による転写因子FOXO1の機能調節と細胞増殖の制御, シンポジウム (2011)
  • Author(s): 坂巻純一, 大徳浩照, 吉用賢治, 三輪正直, 深水昭吉
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(シンポジウム1S4a-6)
  • Year and Date: 20110921-24
• [Presentation] ミトコンドリア局在型P5安定発現細胞におけるH2O2誘導性細胞死とエネルギー代謝 (2011)
  • Author(s): 設楽優, 後藤貴宏, 三輪正直, 小宮徹
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20110921-24
• [Presentation] General method to quantify poly(ADP-ribose) and to isolate polyADP-ribosylated proteins in vivo (2011)
  • Author(s): Miwa, M., Eguchi, T., Nodono, H., Hamada, A., Yamashita, S., Fujii, T., Kamemura, K., Komiya, T., Hasegawa, M., Che, F-S., Ikegami, S.
  • Organizer: FASEB Summer Research Conference : NAD Metabolism & Signaling
  • Place of Presentation: Lucca, Italy
  • Year and Date: 20110904-09
• [Presentation] DNA損傷を必要としない新しい中心体増幅経路 (2011)
  • Author(s): 三輪正直
  • Organizer: 第3回中心体研究会
  • Place of Presentation: 東京理科大学, 東京
  • Year and Date: 2011-12-17
• [Presentation] DNA損傷を起こさない化合物による中心体増幅経路の存在 (2011)
  • Author(s): 田中正和, 津田雅貴, 高橋淳, 塚田匡輝, 虫明正敏, 山田真生, 藤澤順一, 三輪正直
  • Organizer: 第58回日本生化学会近畿支部例会
  • Place of Presentation: 関西医科大学, 大阪
  • Year and Date: 2011-05-21