Functional alalysis of clock genes in highly aggressive pancreato-biliary cancer

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590386
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Hirosaki University
• Principal Investigator: KIJIMA Hiroshi 弘前大学, 医学(系)研究科(研究院), 教授 (90204859)
• Co-Investigator(Renkei-kenkyūsha): SATO Fuyuki 弘前大学, 大学院医学研究科, 助教 (60400131) ; MOROHASHI Satoko 弘前大学, 大学院医学研究科, 助教 (90569592)
• Project Period (FY): 2011 – 2013
• Keywords: 癌 / 膵臓 / 時計遺伝子 / 血管新生 / 遺伝子制御

Research Abstract

Pancreato-biliary cancer is one of the most aggressive malignancies of human. Its aggressive phenotypes are thought to be associated with invasive tumor growth via epithelial-mesenchymal transition (EMT), as well as vascular permeation via tumor angiogenesis. Clock genes regulate not only circadian rhythm (biological clock) of human bodies, but also tumor cell growth, EMT, and tumor angiogenesis of the pancreato-biliary cancer.

Research Products

• [Journal Article] Primary tumours modulate innate immune signalling to create pre-metastatic vascular hyperpermeability foci (2013)
  • Author(s): Hiratsuka S, Ishibashi S, Tomita T, Watanabe A, Akashi-Takamura S, Murakami M, Kijima H, Miyake K, Aburatani H, Maru Y
  • Journal Title: Nat Commun Volume: 4 Pages: 1853
  • Peer Reviewed
• [Journal Article] Epithelial cell adhesion molecule (EpCAM) overexpression is correlated with malignant potentials of intraductal papillary mucinous neoplasms (IPMNs) of the pancreas (2013)
  • Author(s): Yonaiyama S, Toyoki Y, Morohashi S, Sakuraba S, Yoshizawa T, Suzuki T, Wu Y, Kijima H, Hakamada K
  • Journal Title: Biomed Res Volume: 34 Pages: 87-95
  • Peer Reviewed
• [Journal Article] Claudin-1 is associated with invasive growth of human pancreatic cancer (2012)
  • Author(s): Kondo J, Sato F, Wu Y, Seino H, Morohashi S, Kijima H
  • Journal Title: Hirosaki Med J Volume: 63 Pages: 127-135
  • Peer Reviewed
• [Journal Article] Laminin-5γ2 chain expression is associated with tumor cell invasiveness and prognosis of lung squamous cell carcinoma (2012)
  • Author(s): Masuda R, Kijima H, Imamura N, Aruga N, Nakazato K, Oiwa K, Nakano T, Watanabe H, Ikoma Y, Tanaka M, Inokuchi S, Iwazaki M
  • Journal Title: Biomed Res Volume: 33 Pages: 309-317
  • Peer Reviewed
• [Journal Article] Clinicopathological significance of laminin-5γ2 chain expression in superficial esophageal cancer (2012)
  • Author(s): Ito E, Ozawa S, Kijima H, Kazuno A, Miyako H, Nishi T, Chino O, Shimada H, Tanaka M, Inoue S, Inokuchi S, Makuuchi H
  • Journal Title: Dis Esophagus Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Tumor budding is a significant indicator of a poor prognosis in lung squamous cell carcinoma patients (2012)
  • Author(s): Masuda R, Kijima H, Imamura N, Aruga N, Nakamura Y, Masuda D, Takeichi H, Kato N, Nakagawa T, Tanaka M, Inokuchi S, Iwazaki M
  • Journal Title: Mol Med Report Volume: 6 Pages: 937-943
  • Peer Reviewed
• [Journal Article] Clinical significance of wall invasion pattern of subserosa-invasive gallbladder carcinoma (2012)
  • Author(s): Okada K, Kijima H, Imaizumi T, Hirabayashi K, Matsuyama M, Yazawa N, Dowaki S, Tobita K, Ohtani Y, Tanaka M, Inokuchi S, Makuuchi H
  • Journal Title: Oncol Rep Volume: 28 Pages: 1531-1536
  • Peer Reviewed
• [Journal Article] The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer (2012)
  • Author(s): Wu Y, Sato F, Yamada T, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Seino H, Morohashi S, Hakamada K, Abiko Y, Kato Y, Kijima H
  • Journal Title: Int J Oncol Volume: 41 Pages: 1337-1346
  • DOI: 10.3892/ijo.2012.1559
  • Peer Reviewed
• [Journal Article] Expression of cytokeratin 34βE12 is a good indicator of tumor progression in esophageal squamous cell carcinoma (2012)
  • Author(s): Ono M, Kijima H, Seino H, Hakamada K, Igarashi Y
  • Journal Title: Biomed Res Volume: 33 Pages: 183-189
  • Peer Reviewed
• [Journal Article] New invasive patterns as a prognostic factor for superficial esophageal cancer (2012)
  • Author(s): Ito E, Ozawa S, Kijima H, Kazuno A, Nishi T, Chino O, Shimada H, Tanaka M, Inoue S, Inokuchi S, Makuuchi H
  • Journal Title: J Gastroenterol Volume: 47 Pages: 1279-1289
  • Peer Reviewed
• [Journal Article] The basic helix-loop-helix transcription factor DEC2 inhibits TGF-β-induced tumor progression in human pancreatic cancer BxPC-3 cells (2012)
  • Author(s): Sato F, Kawamura H, Wu Y, Sato H, Jin D, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Seino H, Morohashi S, Kato Y, Kijima H
  • Journal Title: Int J Mol Med Volume: 30 Pages: 495-501
  • Peer Reviewed
• [Journal Article] BHLH transcription factor DEC2 regulates pro-apoptotic factor Bim in human oral cancer HSC-3 cells (2012)
  • Author(s): Wu Y, Sato F, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Seino H, Morohashi S, Kato Y, Kijima H
  • Journal Title: Biomed Res Volume: 33 Pages: 75-82
  • Peer Reviewed
• [Journal Article] Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver (2012)
  • Author(s): Sato F, Sato H, Jin D, Bhawal UK, Wu Y, Noshiro M, Kawamoto T, Fujimoto K, Seino H, Morohashi S, Kato Y, Kijima H
  • Journal Title: Biochem Biophys Res Commun Volume: 419 Pages: 441-446
  • Peer Reviewed
• [Journal Article] Methylation of the KEAP1 gene promoter region in human colorectal cancer (2012)
  • Author(s): Hanada N, Takahata T, Zhou Q, Ye X, Sun R, Itoh J, Ishiguro A, Kijima H, Mimura J, Itoh K, Fukuda S, Saijo Y
  • Journal Title: BMC Cancer Volume: 12 Pages: 66
  • Peer Reviewed
• [Journal Article] Potential of exogenous cartilage proteoglycan as a new material for cartilage regeneration (2012)
  • Author(s): Ohshika S, Ishibashi Y, Kon A, Kusumi T, Kijima H, Toh S
  • Journal Title: Int Orthop Volume: 36 Pages: 869-877
  • Peer Reviewed
• [Journal Article] Three-dimensional nuclear luminance analysis in well-differentiated adenocarcinoma of the lung (2011)
  • Author(s): Washiya K, Kanno T, Tone K, Kojima K, Kijima H, Watanabe J
  • Journal Title: Acta Cytol Volume: 55 Pages: 350-356
  • Peer Reviewed
• [Journal Article] Cytological difference between benignity and malignancy in suspicious cases employing urine cytodiagnosis using a liquid-based method (2011)
  • Author(s): Washiya K, Sato T, Miura T, Tone K, Kojima K, Watanabe J, Kijima H
  • Journal Title: Anal Quant Cytol Histol Volume: 33 Pages: 169-174
  • Peer Reviewed
• [Journal Article] Efficacy of Adipose Tissue-Derived Mesenchymal Stem Cells for Fulminant Hepatitis in Mice Induced by Concanavalin A (2011)
  • Author(s): Kubo N, Narumi S, Kijima H, Mizukami H, Yagihashi S, Hakamada K, Nakane A
  • Journal Title: J Gastroenterol Hepatol Volume: Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Histopathological phenotypes of early gastric cancer and its background mucosa (2011)
  • Author(s): Kudo Y, Morohashi S, Takasugi K, Tsutsumi S, Ogasawara H, Hanabata N, Yoshimura T, Sato F, Fukuda S, Kijima H
  • Journal Title: Biomed Res Volume: 32 Pages: 127-134
  • Peer Reviewed
• [Journal Article] Basic helix-loop-helix transcription factor DEC1 negatively regulates cyclin D1 (2011)
  • Author(s): Bhawal UK, Sato F, Arakawa Y, Fujimoto K, Kawamoto T, Tanimoto K, Ito Y, Sasahira T, Sakurai T, Kobayashi M, Kashima I, Kijima H, Kuniyasu H, Abiko Y, Kato Y, Sato S
  • Journal Title: J Pathol Volume: 224 Pages: 420-429
  • Peer Reviewed
• [Journal Article] PERIOD1 (PER1) has anti-apoptotic effects, and PER3 has pro-apoptotic effects during cisplatin (CDDP) treatment in human gingival cancer CA9-22 cells (2011)
  • Author(s): Sato F, Wu Y, Bhawal UK, Liu Y, Imaizumi T, Morohashi S, Kato Y, Kijima H
  • Journal Title: Eur J Cancer Volume: 47 Pages: 1747-1758
  • Peer Reviewed
• [Journal Article] Mesenchymal stromal cells promote tumor growth through the enhancement of neovascularization (2011)
  • Author(s): Suzuki K, Sun R, Origuchi M, Kanehira M, Takahata T, Itoh J, Umezawa A, Kijima H, Fukuda S, Saijo Y
  • Journal Title: Mol Med Volume: 17 Pages: 579-587
  • Peer Reviewed
• [Journal Article] L1 Cell adhesion molecule (L1CAM) expression at the cancer invasive front is a novel prognostic marker of pancreatic ductal adenocarcinoma (2011)
  • Author(s): Tsutsumi S, Morohashi S, Kudo Y, Akasaka H, Ogasawara H, Ono M, Takasugi K, Ishido K, Hakamada K, Kijima H
  • Journal Title: J Surg Oncol Volume: 103 Pages: 669-673
  • Peer Reviewed
• [Journal Article] Basic helix-loop-helix transcription factors DEC1 and DEC2 regulate the paclitaxel-induced apoptotic pathway of MCF-7 human breast cancer cells (2011)
  • Author(s): Wu Y, Sato F, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Morohashi S, Kato Y, Kijima H
  • Journal Title: Int J Mol Med Volume: 27 Pages: 491-495
  • Peer Reviewed
• [Journal Article] Basic-helix-loop-helix transcription factor DEC2 constitutes negative feedback loop in IFN-β-mediated inflammatory responses in human mesangial cells (2011)
  • Author(s): Imaizumi T, Sato F, Tanaka H, Matsumiya T, Yoshida H, Yashiro-Aizawa T, Tsuruga K, Hayakari R, Kijima H, Satoh K
  • Journal Title: Immunol Lett Volume: 136 Pages: 37-43
  • Peer Reviewed
• [Presentation] 胆嚢の前癌病変および早期病変に関する病理形態学的解析 (2013)
  • Author(s): 羽賀敏博, 吉澤忠司, 諸橋聡子, 呉雲燕, 清野浩子, 鬼島宏
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2013-06-08
• [Presentation] 胆嚢の前癌病変・早期癌の病理.(ワークショップ:膵臓・胆道癌の発癌機構と病理診断の展望) (2013)
  • Author(s): 鬼島宏, 吉澤忠司, 呉雲燕, 羽賀敏博, 清野浩子, 諸橋聡子
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2013-06-06
• [Presentation] bHLH transcription factor DEC2 inhibits TGF-β-induced tumor progression in BxPX-3 cells (2013)
  • Author(s): 呉雲燕, 清野浩子, 鈴木貴弘, 諸橋聡子, 吉澤忠司, 加藤幸夫, 鬼島宏
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2013-06-06
• [Presentation] Pathological characteristics of early to advanced gallbladder carcinoma and extrahepatic cholangiocarcinoma (2013)
  • Author(s): Kijima H, Wu Y, Yoshizawa T, Suzuki T, Haga T, Seino H, Morohashi S
  • Organizer: International Symposium on Cholangiocarcinoma
  • Place of Presentation: 東京
  • Year and Date: 2013-02-09
• [Presentation] DEC1 has pro-apoptotic, and DEC2 has anti-apoptotic effects in paclitaxel-treated MCF-7 cells (2012)
  • Author(s): Wu Y, Sato F, Tugeno Y, Morohashi S, Kato Y, Kijima H
  • Organizer: 第8回日本病理学会カンファレンス
  • Place of Presentation: 松本
  • Year and Date: 2012-08-05
• [Presentation] 腫瘍における血管新生因子の発現調節 (2012)
  • Author(s): 鬼島宏
  • Organizer: (レクチャー3)第9回日本病理学会カンファレンス
  • Place of Presentation: 山口
  • Year and Date: 2012-08-03
• [Presentation] Basic helix-loop-helix transcription factor DEC2 negatively regulates pro-apoptotic factor Bim in cisplatin-treated human oral cancer cells (2012)
  • Author(s): Wu Y, Seino H, M orohashi S, Yoshizawa T, Kato Y, Kijima H
  • Organizer: 第9回日本病理学会カンファレンス
  • Place of Presentation: 山口
  • Year and Date: 2012-08-03
• [Presentation] PERIOD is involved in the proliferation of human pancreatic MIA-PaCa2 cancer cells (2012)
  • Author(s): Suzuki T, Wu Y, Seino H, Morohashi S, Yoshizawa T, Haga T, Kato Y, Kijima H
  • Organizer: 第9回日本病理学会カンファレンス
  • Place of Presentation: 山口
  • Year and Date: 2012-08-03
• [Presentation] Anti-apoptotic effect of claudin-1 in tamoxifen-treated breast cancer MCF-7 cells (2011)
  • Author(s): 佐藤冬樹, 赤坂治枝, 諸橋聡子, 呉雲燕, 鬼島宏
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-04-28
• [Presentation] Pro- and anti-apoptotic effects of DEC1/DEC2 in paclitaxel-treated human breast cancer MCF-7 cells (2011)
  • Author(s): 呉雲燕, 佐藤冬樹, 諸橋聡子, 加藤幸夫, 鬼島宏
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-04-28
• [Remarks] 弘前大学大学院医学研究科病理生命科学講座
  • URL: http://www.med.hirosaki-u.ac.jp/~patho2/