2013 Fiscal Year Final Research Report
Analyses of molecular bases of cancer stem cells and their differentiation in Hodgkin lymphoma
| Project/Area Number |
23590432
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HORIE Ryouichi 北里大学, 医学部, 准教授 (80229228)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ホジキンリンパ腫 / 癌幹細胞 / ニッチ / 細胞分化 / 分子標的療法 |
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| Research Abstract |
We identified side population (SP fraction), which is thought to represent cancer stem cell like population and non-SP fraction, which is thought to represent differentiated cells in Hodgkin lymphoma cell lines, KMH2 and L428. SP consists of small cells, whereas non-SP contains relatively large mono nucleated cells and Hodgkin, Reed-Sternberg (H-RS) cells. CD30, a marker for HL is strongly expressed on both SP and non-SP. Both fractions also revealed strong activation of NF-kB. We sorted SP and non-SP and examined expression of mRNA using microarray analyses. The results indicated that molecules related to anti-oxidative stress are more frequently expressed in SP than non-SP. We also found that multi-nucleated cells are generated by abortive mitosis.
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Research Products
(26 results)
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[Journal Article] Incidence and Clinical Significance of Aberrant T-Cell Marker Expression on Diffuse Large B-Cell Lymphoma Cells2013
Author(s)
Suzuki Y, Yoshida T, Wang G, Aoki T, Katayama T, Miyamoto S, Miyazaki K, Iwabuchi K, Danbara M, Nakayama M, Horie R, Nakamine H, Sato Y, Nakamura N, Niitsu N
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Journal Title
Acta Haematol
Volume: 130
Pages: 230-237
DOI
Peer Reviewed
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[Journal Article] Association of CD20 levels with clinicopathological parameters and its prognostic significance for patients with DLBCL2012
Author(s)
Suzuki Y, Yoshida T, Wang G, Togano T, Miyamoto S, Miyazaki K, Iwabuchi K, Nakayama M, Horie R, Niitsu N, Sato Y, Nakamura N
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Journal Title
Ann Hematol
Volume: 91(7)
Pages: 997-1005
DOI
Peer Reviewed
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[Journal Article] Re-appearance of hepatitis B virus following therapy with rituximab for lymphoma is not rare in Japanese patients with past hepatitis B virus infection2011
Author(s)
Watanabe M, Shibuya A, Tsunoda Y, Danbara M, Ishii R, Ohsaka M, Takada J, Tanaka Y, Okuwaki Y, Minamino T, HidakaH, Nakazawa T, Horie R, Higashihara M, Koizumi W
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Journal Title
Liver Int.
Volume: 31(3)
Pages: 340-7
DOI
Peer Reviewed
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[Presentation] he regulation of ''inflammatory niche'' with tumor derived small RNAs2013
Author(s)
山川奈津子, 横山和明, Jun Lu, 今留謙一, 渡辺俊樹, 堀江良一, 穂積勝人, 八幡崇, 安藤潔, 中村直哉, 幸谷愛
Organizer
第75回日本血液学会学術集会
Place of Presentation
札幌
Year and Date
2013-10-11
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