Identification of novel causative genes in mesothelioma and the elucidation of the carcinogenic mechanism

2014 Fiscal Year Final Research Report

• Project/Area Number: 23590464
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Shimane University
• Principal Investigator: KAKAZU Naoki 島根大学, 医学部, 准教授 (20264757)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 中皮腫 / 融合転写産物

Outline of Final Research Achievements

Mesothelioma is a poor prognosis tumor due to former exposure to asbestos. We analyzed mesothelioma cells by next generation sequencing to identify novel candidate fusion genes associated with the development of mesothelioma. Firstly, we performed whole transcriptome shotgun sequencing (RNA-Seq) of a mesothelioma cell line and comprehensively detected many fusion transcripts. Secondly, we performed RT-PCR in the cell line and the other five to confirm the presence of these detected fusion transcripts, and narrowed down the candidate fusion genes. These results suggest that two fusion genes (DUS4L-BCAP29, PDPN-PRDM2) are the potential candidates implicated in the pathogenesis of mesothelioma in this study.

Free Research Field

細胞遺伝学、分子生物学