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2013 Fiscal Year Final Research Report

Development of the expression system of helminthic mitochondrial quinol-fumarate reductase in bacteria.

Research Project

  • PDF
Project/Area Number 23590484
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionHirosaki University

Principal Investigator

SAKAMOTO Kimitoshi  弘前大学, 農学生命科学部, 准教授 (50361465)

Project Period (FY) 2011 – 2013
Keywords蠕虫 / ミトコンドリア呼吸鎖
Research Abstract

Quinol-fumarate reductase (QFR) is the key enzyme for the anaerobic respirtory chain of hypoxia adapted helminthes. QFR is characteristic by its effective catalytic activity for the reverse reaction of aerobic respiratory complex II. In the aim of elucidating the mechanisms of enhancing reverse reaction, the development of expression system in bacteria was planned. At first, all the genes for the subunits of QFR from Echinococcus multilocularis were cloned. N terminal amino acid of them were also determined from the QFR in mitochondria. Complex II gene from the expression host, Rhodospirillum rubrum was knocked out. Finally, plasmid for expressing Echonococcus QFR was prepared.

  • Research Products

    (8 results)

All 2013 2012 2011

All Journal Article (5 results) Presentation (3 results)

  • [Journal Article] Pharmacophore identification of ascofuranone, potent inhibitor of cyanide-insensitive alternative oxidase of Trypanosoma brucei2013

    • Author(s)
      Saimoto H, Kido Y, Haga Y, Sakamoto K, Kita K
    • Journal Title

      The Journal of Biochemistry

      Volume: 153 Pages: 267-273

  • [Journal Article] Structure of the trypanosome cyanide-insensitive alternative oxidase2013

    • Author(s)
      Shiba T, Kido Y, Sakamoto K, Inaoka DK, Tsuge C, Tatsumi R, Takahashi G, Balogun EO, Nara T, Aoki T, Honma T, Tanaka A, Inoue M, Matsuoka S, Saimoto H, Moore AL, Harada S, Kita K
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 110 Pages: 4580-4585

  • [Journal Article] Age-related changes in the activities of respiratory chain complexes and mitochondrial morphology in Drosophila2012

    • Author(s)
      Oda Y, Yui R, Sakamoto K, Kita K, Matsuura ET
    • Journal Title

      Mitochondrion

      Volume: 12 Pages: 345-351

  • [Journal Article] Crystal structure of mitochondrial quinol-fumarate reductase from the parasitic nematode Ascaris suum2012

    • Author(s)
      Shimizu H, Osanai A, Sakamoto K, Inaoka D K, Shiba T, Harada S, Kita K
    • Journal Title

      The Journal of Biochemistry

      Volume: 151 Pages: 589-592

  • [Journal Article] Ukulactones A and B, new NADH-fumarate reductase inhibitors produced by Penicillium sp. FKI-33892011

    • Author(s)
      Mori M, Morimoto H, Kim YP, Ui H, Nonaka K, Masuma R, Sakamoto K, Kita K, Tomoda H, Shiomi K, Omura S
    • Journal Title

      Tetrahedron

      Volume: 67 Pages: 6582-6586

  • [Presentation] Rhodobacter capsulatusとRhodospirillum rubrum間でのキメラ型コハク酸‐ユビキノン還元酵素の作製2013

    • Author(s)
      福士実咲・柴谷恵太・Hendri Aldrat・北潔・Fevzi Daldal・坂元君年
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜・横浜
    • Year and Date
      20130000
  • [Presentation] 紅色光合成細菌Rhodobacter capsulatusを用いたコハク酸脱水素酵素複合体の異種生物間発現2012

    • Author(s)
      坂元君年・Hendri Aldrat・北潔
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      20120000
  • [Presentation] コハク酸脱水素酵素複合体の異種生物間発現系の開発2011

    • Author(s)
      坂元君年・Hendri Aldrat・北潔
    • Organizer
      日本農芸化学会東北支部第146回大会
    • Place of Presentation
      山形大学
    • Year and Date
      20110000

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Published: 2015-07-16  

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