Characterization for secreted egg proteins associate with development of post schistosomal fibrosis.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590489
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Nagasaki University
• Principal Investigator: KIKUCHI Mihoko 長崎大学, 熱帯医学研究所, 講師 (40336186)
• Co-Investigator(Kenkyū-buntansha): HIRAYAMA Kenji 長崎大学, 熱帯医学研究所, 教授 (60189868)
• Project Period (FY): 2011 – 2013
• Keywords: 日本住血吸虫 / 肝線維化症 / 虫卵由来組み換え蛋白 / 新規蛋白機能解析 / SEA-domein

Research Abstract

While isolating membrane-bound and secreted proteins as targets for Schistosoma japonicum vaccine, we identified a novel potentially functional gene family which had originated by a gene duplication mechanism. Here, we integrated structural homology modeling and biochemical methods to show that this gene family encodes proteins with sea-urchin sperm protein, enterokinase and agrin (SEA)domain, with heme-binding properties. Typical of SEA-structural domains, the characterized proteins specifically interacted with glycosaminoglycans, with implication in ligand gathering and immune-evasion. Consistent with modeled heme-binding pocket, we observed high affinity heme-binding and spectroscopic attributes of hexa-coordinated heme iron. Localization of the native gene-products on adult worm tegument andgastrodermis, host interfaces for heme-sequestration and acquisition, suggests potential roles for this gene family in heme-detoxification and heme-iron uptake.

Research Products

• [Journal Article] Detection of active schistosome infection by cell-free circulatin DNA of Schistosoma japonicum in highly endemic areas in Sorsogon Province, the Philippines (2014)
  • Author(s): Kato-Hayashi N, Leonardo LR, Arevalo NL, Tagum MN, Apin J, Agsolid LM, Chua JC, Villacorte EA, Kirinoki M, Kikuchi M, Ohmae H, Haruki K, Chigusa Y
  • Journal Title: Acta Trop Volume: (pii : S0001-706X(14)00168-5)
  • DOI: 10.1016/j.actatropica
  • Peer Reviewed
• [Journal Article] Characterization of a gene family encoding SEA (sea-urchin sperm protein, enterokinase and agrin)-domain proteins with lectin-like and heme-binding properties from Schistosoma japonicum (2014)
  • Author(s): Mbanefo EC, Kikuchi M, Huy NT, Shuaibu MN, Cherif MS, Yu C, Wakao M, Suda Y, Hirayama K
  • Journal Title: PLoS Negl Trop Dis Volume: 8(1) Pages: e2644
  • DOI: 10.1371/journal.pntd.0002644
  • Peer Reviewed
• [Journal Article] Genetic Structure Inferred from Mitochondrial 12S Ribosomal RNA Sequence of Oncomelaniaquadrasi, the Intermediate Snail Host of Schistosoma japonicum in the Philippines (2014)
  • Author(s): Weerachai Saijuntha, Blanca Jarilla, Alvin K. Leonardo, Louie S. Sunico, Lydia R. Leonardo, Ross H. Andrews, Paiboon Sithithaworn, Trevor N. Petney, Masashi Kirinoki, Naoko Kato-Hayashi, Mihoko Kikuchi, Yuichi Chigusa, and Takeshi Agatsuma
  • Journal Title: American Jornal of Tropical Medicine and Hygene Volume: (Epub ahead of print)
  • DOI: 10.4269/ajtmh.13-0260
  • Peer Reviewed
• [Journal Article] Origin of a novel protein-coding gene family with similar signal sequence in Schistosoma japonicum (2012)
  • Author(s): Mbanefo EC, Chuanxin Y, Kikuchi M, Shuaibu MN, Boamah D, Kirinoki M, Hayashi N, Chigusa Y, Osada Y, Hamano S, Hirayama K
  • Journal Title: BMC Genomics Volume: 13 Pages: 260
  • DOI: 10.1186/1471-2164-13-260
  • Peer Reviewed
• [Journal Article] Association of HLA and post-schistosomal hepatic disorder : A systematic review and meta-analysis (2011)
  • Author(s): Huy NT, Hamada M, Kikuchi M, Lan NT, Yasunami M, Zamora J, Hirayama K
  • Journal Title: Parasitol Int Volume: 60(4) Pages: 347-356
  • DOI: 10.1016
  • Peer Reviewed
• [Presentation] Characterization of a novel SEA-domain protein family with lectin-like and heme-binding properties from Schistosoma japonicum (2013)
  • Author(s): Mbanefo EC, Kikuchi M, Huy NT, Shuaibu MN, Cherif MS, Chuanxin Y, Suda Y, Hirayama K
  • Organizer: The 12th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji Yumebutai International Conference Center, Awaji City, Hyogo, Japan
  • Year and Date: 20130910-13
• [Presentation] Origin of novel protein-coding genes with similar signal sequence in Schistosoma japonicum (2012)
  • Author(s): Evaristus CM, Kikuchi M., et al.
  • Organizer: 日本熱帯医学会
  • Place of Presentation: 北海道とかちプラザ
  • Year and Date: 20120905-06
• [Presentation] Surveyllance on Schistosomal Fibrosis in Sorsogon Province, the Phillippins (2012)
  • Author(s): 菊池三穂子他
  • Organizer: 日本熱帯医学会
  • Place of Presentation: 北海道とかちプラザ
  • Year and Date: 20120905-06
• [Presentation] Immunogenetic analysis of the patients with early-onset of schistosomal liver fibrosis in Sorsogon Province, the Philippines (2012)
  • Author(s): Kikuchi. M. et al.
  • Organizer: Forum Cheju 15
  • Place of Presentation: 宮崎宮崎パームビーチホテル
  • Year and Date: 20120523-25
• [Presentation] Immunogenetic analysis of the patients with early-onset of schistosomal liver fibrosis in Sorsogon Province, the Philippines (2012)
  • Author(s): Kikuchi. M. et al.
  • Organizer: 5th The ASEAN Congress of Tropical Medicine and Parasitology (ACTMP)
  • Place of Presentation: anila, Philippine, University of Philippine
  • Year and Date: 20120515-17
• [Presentation] Immunogenetic analysis of the patients with early onset schistosomal fibrosis in Sorsogon Province, the Philippines (2011)
  • Author(s): Kikuchi M, et al.
  • Organizer: 45th Annual Japan-U.S. Joint Conference on Parasitic Diseases, Japan-US Parasitic Diseases Panel Meeting, Japan-US Cooperative Medical Science Program
  • Place of Presentation: 東京、東京大学
  • Year and Date: 20110110-11
• [Book] 感染症事典, 住血吸虫 (2012)
  • Author(s): 感染症事典編集委員会編, 共著菊池三穂子
  • Total Pages: 617(527-532)
  • Publisher: オーム社