2013 Fiscal Year Final Research Report
Development of human monoclonal antibodies to major surface antigens of parasitic protozoa for clinical applications
| Project/Area Number |
23590496
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Tokai University |
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Principal Investigator |
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| Research Collaborator |
CHENG Xunjia 復旦大学, 医学院, 教授
FU Yongfeng 東海大学, 医学院, 研究員
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| Project Period (FY) |
2011 – 2013
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| Keywords | 寄生原虫 / ヒトモノクローナル抗体 / 熱帯熱マラリア / 赤痢アメーバ症 / 抗体遺伝子 / 抗体医薬 / 表面抗原 |
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| Research Abstract |
Whole human monoclonal IgG molecules were prepared from a Fab fragment specific to the C-terminus 19-kDa fragment of Plasmodium falciparum merozoite surface protein 1. Affinities of IgG1 and IgG3 molecules produced in CHO cells were approximately 20-fold higher than that of the original Fab fragment. Whole human IgG1 molecule was also prepared from a monoclonal antibody Fab fragment specific for surface lectin of Entamoeba histolytica. These whole human IgG molecules may be applicable for prevention, diagnosis and therapy of protozoan infectious diseases.
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