Effect of a potential Chlamydial effecter protein, CpB0850 on cell cycle progression of host cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590505
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: University of the Ryukyus (2013); Osaka University (2011-2012)
• Principal Investigator: HIRAI Itaru 琉球大学, 医学部, 教授 (00359994)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Yoshimasa 大阪府立公衆衛生研究所, 所長 (20010100)
• Project Period (FY): 2011 – 2013
• Keywords: 遺伝子 / 細菌 / 微生物

Research Abstract

In this study, we examined effect of Chlamydophila pneumoniae infection on host cells. Cell growth of host cells were suppressed by C. pneumoniae infection through modification of certain signal transduction pathway. In addition, it was suggested that a potential Chlamydial effector protein, CpB0850 might effect on cell cycle progression through interaction with host cellular chromosomal protein.

Research Products

• [Journal Article] Detection of chromosomal blaCTX-M-15 in Escherichia coli O25b-B2-ST131 isolates from the Kinki region of Japan (2013)
  • Author(s): Hirai I, Fukui N, Taguchi M, Yamauchi K, Nakamura T, Okano S, Yamamoto Y
  • Journal Title: Int J Antimicrob Agents Volume: 42(6) Pages: 500-6
  • Peer Reviewed
• [Journal Article] Fecal carriage of CTX-Mβ-lactamase-producing Enterobacteriaceae in nursing homes in the Kinki region of Japan (2013)
  • Author(s): Luvsansharav UO, Hirai I, Niki M, Nakata A, Yoshinaga A, Yamamoto A, Yamamoto M, Toyoshima H, Kawakami F, Matsuura N, Yamamoto Y
  • Journal Title: Infect Drug Resist Volume: 22;6 Pages: 67-70
  • Peer Reviewed
• [Journal Article] Dengue virus neutralization and antibody-dependent enhancement activities of human monoclonal antibodies derived from dengue patients at acute phase of secondary infection (2013)
  • Author(s): Sasaki T, Setthapramote C, Kurosu T, Nishimura M, Asai A, Omokoko MD, Pipattanaboon C, Pitaksajjakul P, Limkittikul K, Subchareon A, Chaichana P, Okabayashi T, Hirai I, Leuangwutiwong P, Misaki R, Fujiyama K, Ono KI, Okuno Y, Ramasoota P, Ikuta K
  • Journal Title: Antiviral Res Volume: 98(3) Pages: 423-31
  • Peer Reviewed
• [Journal Article] Chlamydophila pneumoniae in human immortal Jurkat cells and primary lymphocytes uncontrolled by interferon-γ (2013)
  • Author(s): Ishida K, Kubo T, Saeki A, Yamane C, Matsuo J, Yimin, Nakamura S, Hayashi Y, Kunichika M, Yoshida M, Takahashi K, Hirai I, Yamamoto Y, Shibata K, Yamaguchi H
  • Journal Title: Microbes Infect Volume: 15(3) Pages: 192-200
  • Peer Reviewed
• [Journal Article] Jurkat cell proliferation is suppressed by Chlamydia (Chlamydophila) pneumoniae infection accompanied with attenuation of phosphorylation at Thr389 of host cellular p70S6 K (2013)
  • Author(s): Hirai I, Ebara M, Nakanishi M, Yamamoto C, Sasaki T, Ikuta K, Yamamoto Y
  • Journal Title: Immunobiology Volume: 218(4) Pages: 527-532
  • Peer Reviewed
• [Journal Article] Human monoclonal antibodies to neutralize all dengue virus serotypes using lymphocytes from patients at acute phase of the secondary infection (2013)
  • Author(s): Setthapramote C, Sasaki T, Puiprom O, Limkittikul K, Pitaksajjakul P, Pipattanaboon C, Sasayama M, Leuangwutiwong P, Phumratanaprapin W, Chamnachanan S, Kusolsuk T, Jittmittraphap A, Asai A, Arias JF, Hirai I, Kuhara M, Okuno Y, Kurosu T, Ramasoota P, Ikuta K
  • Journal Title: Biochem Biophys Res Commun Volume: 432(1) Pages: 200-1
  • Peer Reviewed
• [Journal Article] Prevalence of and risk factors associated with fecal carriage of CTX-Mβ-lactamase-producing Enterobacteriaceae in rural Thai communities (2012)
  • Author(s): Luvsansharav UO, Hirai I, Nakata A, Imura K, Yamauchi K, Niki M, Komalamisra C, Kusolsuk T, Yamamoto Y
  • Journal Title: J Antimicrob Chemother Volume: 67(7) Pages: 1769-74
  • Peer Reviewed
• [Journal Article] Chlamydia trachomatis serovar L2 infection model using human lymphoid Jurkat cells (2012)
  • Author(s): Kubo T, Ishida K, Matsuo J, Nakamura S, Hayashi Y, Sakai H, Yoshida M, Takahashi K, Hirai I, Yamamoto Y, Yamaguchi Y
  • Journal Title: Microb Pathog Volume: 53(1) Pages: 1-11
  • Peer Reviewed
• [Journal Article] Epidemiology of Escherichia coli, Klebsiella Species, and Proteus mirabilis Strains Producing Extended-Spectrumβ-Lactamases From Clinical Samples in the Kinki Region of Japan (2012)
  • Author(s): Nakamura T, Komatsu M, Yamasaki K, Fukuda S, Miyamoto Y, Higuchi T, Ono T, Nishio H, Sueyoshi N, Kida K, Satoh K, Toda H, Toyokawa M, Nishi I, Sakamoto M, Akagi M, Nakai I, Kofuku T, Orita T, Wada Y, Zikimoto T, Koike C, Kinoshita S, Hirai I, Takahashi H, Matsuura N, Yamamoto Y
  • Journal Title: Am J Clin Pathol Volume: 137(4) Pages: 620-6
  • Peer Reviewed
• [Journal Article] Extended-spectrumβ-lactamase-producing Escherichia coli strains in the feces of carriers contribute substantially to urinary tract infections in these patients (2011)
  • Author(s): Niki M, Hirai I, Yoshinaga A, Ulzii-Orshikh L, Nakata A, Yamamoto A, Yamamoto M, Yamamoto Y
  • Journal Title: Infection Volume: 39(5) Pages: 467-71
  • Peer Reviewed
• [Journal Article] 医療系学生における基質特異性拡張型βラクタマーゼ産生腸内細菌保持率 (2011)
  • Author(s): 仁木真理恵,平井到,山本容正
  • Journal Title: 日本環境感染学会誌 Volume: 26(3) Pages: 154-156
  • Peer Reviewed
• [Journal Article] Chlamydophila pneumoniae attachment and infection in low proteoglycan expressing human lymphoid Jurkat cells (2011)
  • Author(s): Kobayashi M, Ishida K, Matsuo J, Nakamura S, Nagasawa A, Motohashi K, Yao T, Hirai I, Yamamoto Y, Suzuki H, Shimizu C, Matsuno K, Yamaguchi H
  • Journal Title: Microb Pathog Volume: 51(3) Pages: 209-16
  • Peer Reviewed
• [Journal Article] Analysis of risk factors for a high prevalence of extended-spectrumβ-lactamase-producing Enterobacteriaceae in asymptomatic individuals in rural Thailand (2011)
  • Author(s): Luvsansharav UO, Hirai I, Niki M, Sasaki T, Makimoto K, Komalamisra C, Maipanich W, Kusolsuk T, Sa-Nguankiat S, Pubampen S, Yamamoto Y
  • Journal Title: J Med Microbiol Volume: 60(Pt 5) Pages: 619-24
  • Peer Reviewed
• [Journal Article] Prevalence of fecal carriage of extended-spectrumβ-lactamase-producing Enterobacteriaceae among healthy adult people in Japan (2011)
  • Author(s): Luvsansharav UO, Hirai I, Niki M, Nakata A, Yoshinaga A, Moriyama T, Yamamoto Y
  • Journal Title: J Infect Chemother Volume: 17(5) Pages: 722-5
  • Peer Reviewed
• [Presentation] Dissemination of CTX-M type ESBL-producing bacteria in Southeast Asian countries (2014)
  • Author(s): Itaru Hirai
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
  • Year and Date: 20140326-28
• [Presentation] High Prevalence of Extended Spectrumβ-lactamase-producing Escherichia coli in Household Members of a Rural Area in Vietnam (2013)
  • Author(s): H. Bui, I. Hirai, T. Nguyen, V. Ha, N. Bui, H. Phan, T. Le, S. Ueda, H. Watabe, H. Le, Y. Yamamoto
  • Organizer: 53rd ICAAC
  • Place of Presentation: Denver, CO
  • Year and Date: 20130910-13
• [Presentation] 健常人由来CTX-M型ESBL産生菌株におけるESBL遺伝子の存在形態と地理的分布の関連性 (2013)
  • Author(s): 平井到,山内紘,山本容正
  • Organizer: 第86回日本西院学会総会
  • Place of Presentation: 千葉市
  • Year and Date: 20130318-20
• [Presentation] A sequencing analysis of genetic environment surrounding blaCTX-M-15 in Escherichia coli (2012)
  • Author(s): Naoki Fukui, Itaru Hirai, Arisa Nakata, Aya Yoshinaga, Ulzii-Orshikh Luvsansharav, Tatsuya Nakamura, Yoshimasa Yamamoto
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 20120327-29
• [Presentation] A 2-year trend of CTX-M beta-lactamase-producing Enterobacteriaceae in asymptomatic Thai people (2012)
  • Author(s): Ulzii-Orshikh Luvsansharav, Itaru Hirai, Arisa Nakata, Kaori Imura, Kou Yamauchi, Marie Niki, Chalit Komalamisra, Teera Kusolsuk, Yoshimasa Yamamoto
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 20120327-29
• [Presentation] CELL DIVISION OF INFECTED CELLS IS RETARDED BY SECRETION OF A NOVEL EFFECTER PROTEIN, CPB0850, OF CHLAMYDIA (CHLAMYDOPHILA) PNEUMONIAE (2011)
  • Author(s): Itaru Hirai, Masanori Matsuba, Yoshimasa Yamamoto
  • Organizer: The IUMS 2011 Sapporo Congress
  • Place of Presentation: 札幌
  • Year and Date: 20110906-10