Mechanism of bacterial ATP secretion.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590521
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Jikei University School of Medicine
• Principal Investigator: MIZUNOE Yoshimitsu 東京慈恵会医科大学, 医学部, 教授 (20157514)
• Co-Investigator(Kenkyū-buntansha): TAJIMA Akiko 東京慈恵会医科大学, 医学部, 講師 (70317973) ; IWASE Tadayuki 東京慈恵会医科大学, 医学部, 講師 (80385294) ; SUGIMOTO Shinya 東京慈恵会医科大学, 医学部, 講師 (60464393)
• Project Period (FY): 2011 – 2013
• Keywords: ATP / グルコース / 解糖系 / 増殖期 / 細菌

Research Abstract

ATP modulates immune cell functions. We recently reported that Enterococcus gallinarum, isolated from mice and humans, secretes ATP. We have since found and characterized several ATP-secreting bacteria. Of the tested enterococci, Enterococcus mundtii secreted the greatest amount of ATP after overnight culture. Glucose, not amino acids and vitamins, was essential for ATP secretion from E. mundtii. Analyses of energy-deprived cells demonstrated that glycolysis is the most important pathway for bacterial ATP secretion. Furthermore, exponential-phase E. mundtii and Enterococcus faecalis cells secrete ATP more efficiently than stationary-phase cells. Other bacteria, including Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, also secrete ATP in exponential but not stationary phase. These results suggest that various gut bacteria, including commensals and pathogens, might secrete ATP at any growth phase and modulate immune cell function.

Research Products

• [Journal Article] Immuno-electron microscopy of primary cell cultures from genetically modified animals in liquid by atmospheric scanning electron microscopy (2014)
  • Author(s): Kinoshita T, Mori Y, Hirano K, Sugimoto S, Okuda K, Matsumoto S, Namiki T, Ebihara T, Kawata M, Nishiyama H, Sato M, Suga M, Higashiyama K, Sonomoto K, Mizunoe Y, Nishihara S, Sato C
  • Journal Title: Microsc Microanal Volume: 20(2) Pages: 470-84
  • DOI: 10.1017/S1431927614000178
  • Peer Reviewed
• [Journal Article] Effects of Bacteriocins on Methicillin-Resistant Staphylococcus aureus Biofilm (2013)
  • Author(s): Okuda K, Zendo T, Sugimoto S, Iwase T, Tajima A, Yamada S, Sonomoto K, Mizunoe Y
  • Journal Title: Antimicrob Agents Chemother Volume: 57(11) Pages: 5572-9
  • DOI: 10.1128/AAC.00888-13
  • Peer Reviewed
• [Journal Article] A simple assay for measuring catalase activity : a visual approach (2013)
  • Author(s): Iwase T, Tajima A, Sugimoto S, Okuda K, Hironaka I, Kamata Y, Takada K, Mizunoe Y
  • Journal Title: Sci Rep Volume: 3 Pages: 3081
  • DOI: 10.1038/srep03081
  • Peer Reviewed
• [Journal Article] Glucose triggers ATP secretion from bacteria in a growth-phase-dependent manner (2013)
  • Author(s): Hironaka I, Iwase T, Sugimoto S, Okuda K, Tajima A, Yanaga K, Mizunoe Y
  • Journal Title: Appl Environ Microbiol Volume: 79(7) Pages: 2328-35
  • DOI: 10.1128/AEM.03871-12
  • Peer Reviewed
• [Journal Article] Staphylococcus epidermidis Esp degrades specific proteins associated with Staphylococcus aureus biofilm formation and host-pathogen interaction (2013)
  • Author(s): Sugimoto S, Iwamoto T, Takada K, Okuda K, Tajima A, Iwase T, Mizunoe Y
  • Journal Title: J Bacteriol Volume: 195(8) Pages: 1645-55
  • DOI: 10.1128/JB.01672-12
  • Peer Reviewed
• [Journal Article] Purification, crystallization and preliminary X-ray diffraction analysis of the Staphylococcus epidermidis extracellular serine protease Esp (2013)
  • Author(s): Vengadesan K, Macon K, Sugimoto S, Mizunoe Y, Iwase T, Narayana SV
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun Volume: 69(Pt 1) Pages: 49-52
  • DOI: 10.1107/S1744309112047124
  • Peer Reviewed
• [Journal Article] Campylobacter jejuni DNA-binding protein from starved cells in Guillain-Barré syndrome patients (2011)
  • Author(s): Kawamura N, Piao H, Minohara M, Matsushita T, Kusunoki S, Matsumoto H, Ikenaka K, Mizunoe Y, Kira JI
  • Journal Title: J Neuroimmunol Volume: 240-241 Pages: 74-8
  • DOI: 10.1016/j.jneuroim.2011.09.004
  • Peer Reviewed
• [Journal Article] Cloning, expression and purification of extracellular serine protease Esp, a biofilm degrading enzyme, from Staphylococcus epidermidis (2011)
  • Author(s): Sugimoto S, Iwase T, Sato F, Tajima A, Shinji H, Mizunoe Y
  • Journal Title: J Appl Microbiol Volume: 111(6) Pages: 1406-15
  • DOI: 10.1111/j.1365-2672.2011.05167.x
  • Peer Reviewed
• [Journal Article] Intranasal application of S. epidermidis prevents colonization by methicillin-resistant Staphylococcus aureus in mice (2011)
  • Author(s): Park B, Iwase T, Liu GY
  • Journal Title: PLoS One Volume: 6(10) Pages: e25880
  • DOI: 10.1371/journal.pone.0025880
  • Peer Reviewed
• [Journal Article] Role of FnBPA and FnBPB on in vitro cellular and in vivo septic infections by Staphylococcus aureus (2011)
  • Author(s): Shinji H, Yosizawa Y, Tajima A, Iwase T, Sugimoto S, Seki K, Mizunoe Y
  • Journal Title: Infect Immun Volume: 79(6) Pages: 2215-23
  • DOI: 10.1128/IAI.00133-11
  • Peer Reviewed
• [Journal Article] Positive cooperativity of the p97 AAA ATPase is critical for essential functions (2011)
  • Author(s): Nishikori S, Esaki M, Yamanaka K, Sugimoto S, Ogura T
  • Journal Title: J Biol Chem Volume: 286(18) Pages: 15815-20
  • DOI: 10.1074/jbc.M110.201400
  • Peer Reviewed
• [Presentation] バイオフィルム感染症の予防・治療法の開発 (2014)
  • Author(s): 水之江義充
  • Organizer: 第48回緑膿菌感染症研究会
  • Place of Presentation: 長崎県長崎市
  • Year and Date: 20140324-25
• [Presentation] 大気圧走査電子顕微鏡によるバクテリアの多細胞的形態"バイオフィルム"の液中高分解能観察 (2013)
  • Author(s): 杉本真也,奥田賢一,千葉明生,佐藤主税,水之江義充
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 20131203-06
• [Presentation] 細菌の多細胞的振る舞い"バイオフィルム"の形成機構の解明と制圧に向けた試み (2013)
  • Author(s): 水之江義充
  • Organizer: 第23回日本病態生理学会
  • Place of Presentation: 東京都港区(特別講演)
  • Year and Date: 20130802-04
• [Presentation] Staphylococcus epidermidis esp degrades specific proteins associated with Staphylococcus aureus biofilm formation and host-pathogen interaction (2013)
  • Author(s): Sugimoto S, Iwamoto T, Takada K, Okuda K, Tajima A, Iwase T, Chiba S, Mizunoe Y
  • Organizer: FEMS 2013: 5th congress of European microbiologists
  • Place of Presentation: Leipzig, Germany
  • Year and Date: 20130721-25
• [Presentation] バイオフィルム感染症の制圧をめざして (2013)
  • Author(s): 水之江義充
  • Organizer: 第36回日本骨・関節感染症学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 20130705-06
• [Presentation] 細菌間干渉・細菌宿主相互作用:常在菌による黄色ブドウ球菌の排除 (2013)
  • Author(s): 水之江義充,岩瀬忠行
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉県千葉市(ワークショップ)
  • Year and Date: 20130318-20
• [Presentation] Bacterial ATP secretion (2013)
  • Author(s): Hironaka I, Iwase T, Sugimoto S, Okuda K, Tajima A, Yanaga K, Mizunoe Y
  • Organizer: Annual Conference of the Association for General and Applied Microbiology
  • Place of Presentation: Bremen Germany
  • Year and Date: 20130310-13
• [Presentation] メチシリン耐性黄色ブドウ球菌バイオフィルムに対するバクテリオシンの殺菌効果 (2013)
  • Author(s): 奥田賢一,杉本真也,岩瀬忠行,田嶌亜紀子,水之江義充
  • Organizer: 第27回Bacterial Adherence & Biofilm
  • Place of Presentation: 東京都文京区
  • Year and Date: 2013-07-12
• [Presentation] MRSAのバイオフィルムマトリクスタンパク質の網羅的解析と時空間的動態 (2012)
  • Author(s): 杉本真也,奥田賢一,佐藤主税,水之江義充
  • Organizer: 第95回日本細菌学会関東支部総会
  • Place of Presentation: 東京都港区(シンポジウム)
  • Year and Date: 20121010-12
• [Presentation] Quality control of staphylococcal biofilms by excreted molecular chaperones DnaK and ClpB (2012)
  • Author(s): Sugimoto S, Okuda K, Tajima A, Iwase T, Mizunoe Y
  • Organizer: EMBO/EMBL Symposium, Quality Control～From Molecules to Organelles
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 20120919-22
• [Presentation] 黄色ブドウ球菌のバイオフィルム形成メカニズムの解析 (2012)
  • Author(s): 水之江義充
  • Organizer: 第79回日本細菌学会北海道支部学術総会
  • Place of Presentation: 北海道帯広市(特別講演)
  • Year and Date: 20120828-29
• [Presentation] バイオフィルム研究の最新のアプローチ (2012)
  • Author(s): 水之江義充
  • Organizer: 第23回尿路感染症研究会
  • Place of Presentation: 東京都港区
  • Year and Date: 2012-10-10
• [Presentation] 黄色ブドウ球菌の定着とバイオフィルム形成 (2012)
  • Author(s): 水之江義充
  • Organizer: 第3回MRSAフォーラム
  • Place of Presentation: 東京都新宿区(トピックレクチャー)
  • Year and Date: 2012-07-14
• [Presentation] Proteomic survey for Staphylococcus aureus biofilm matrix proteins : extracellular AAA+ chaperone ClpB contributes to the biofilm formation (2011)
  • Author(s): Sugimoto S, Iwamoto T, Sato F, Tajima A, Iwase T, Shinji H, Mizunoe Y
  • Organizer: 9th international congerence an AAA proteins
  • Place of Presentation: 熊本県熊本市
  • Year and Date: 20111106-11
• [Presentation] Commensal bacterium Staphylococcus epidermidis serine protease esp eliminates Staphylococcus aureus nasal colonization via biofilm destruction (2011)
  • Author(s): Mizunoe Y, Iwase T, Tajima A, Sugimoto S, Sato F, Shinji H
  • Organizer: International union of Microbiological Societies 2011 Congress
  • Place of Presentation: 北海道札幌市
  • Year and Date: 20110906-10
• [Presentation] Efficient and economic extracellular production and purification of Staphylococcus epidermidis Esp using a Brevibacillus choshinensis secretion system (2011)
  • Author(s): Sugimoto S, Iwase T, Sato F, Tajima A, Shinji H, Yoshizawa Y, Mizunoe Y
  • Organizer: International union of Microbiological Societies 2011 Congress
  • Place of Presentation: 北海道札幌市
  • Year and Date: 20110906-10
• [Presentation] 細菌の形成するバイオフィルム (2011)
  • Author(s): 水之江義充
  • Organizer: 第85回日本感染症学会総会・学術講演会
  • Place of Presentation: 東京都港区
  • Year and Date: 20110421-22