2013 Fiscal Year Final Research Report
The analysis of functional domains of NS1 protein involved in the regulation of influenza C virus replication
| Project/Area Number |
23590536
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Yamagata University |
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Principal Investigator |
HONGO SEIJI 山形大学, 医学部, 教授 (90229245)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMOTAI Yoshitaka 山形大学, 医学部, 助教 (30445746)
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| Project Period (FY) |
2011 – 2013
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| Keywords | C型インフルエンザ / ウイルス / NS1 / スプライシング / 核移行シグナル / 核外移行シグナル / 増殖 |
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| Research Abstract |
To investigate the mechanism by which influenza C virus NS1 protein can up-regulate splicing, we tried to find the nuclear localization signal of NS1. The C-terminal deletion mutants of NS1 were expressed in COS-1 cells and the intracellular localization of the expressed mutants was analyzed by immunofluorescence. The wild type NS1 composed of residues 1-246 and C-terminal deletion mutant of residues 1-154 were localized in the cytoplasm predominantly. However, C-terminal deletion mutant of residues 1-108 was localized in the nucleus and cytoplasm. These results suggest that nuclear export signal of NS1 may be present between residues 109 and 154.
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Author(s)
Mizuta K, Abiko C, Aoki Y, Ikeda T, Matsuzaki Y, Hongo S, Itagaki T, Katsushima N, Ohmi A, Nishimura H, Ahiko T.
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Journal Title
Microbiol Immunol
Volume: 57(5)
Pages: 400-5
DOI
Peer Reviewed
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[Journal Article] Intrinsic temperature sensitivity of influenza C virus hemagglutinin-esterase-fusion protein2012
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Takashita E, Muraki Y, Sugawara K, Asao H, Nishimura H, Suzuki K, Tsuji T, Hongo S, Ohara Y, Kawaoka Y, Ozawa M, Matsuzaki Y.
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Pages: 13108-13111
DOI
Peer Reviewed
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