2013 Fiscal Year Final Research Report
The analysis of functional domains of NS1 protein involved in the regulation of influenza C virus replication
Project/Area Number |
23590536
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Virology
|
Research Institution | Yamagata University |
Principal Investigator |
HONGO SEIJI 山形大学, 医学部, 教授 (90229245)
|
Co-Investigator(Kenkyū-buntansha) |
SHIMOTAI Yoshitaka 山形大学, 医学部, 助教 (30445746)
|
Project Period (FY) |
2011 – 2013
|
Keywords | C型インフルエンザ / ウイルス / NS1 / スプライシング / 核移行シグナル / 核外移行シグナル / 増殖 |
Research Abstract |
To investigate the mechanism by which influenza C virus NS1 protein can up-regulate splicing, we tried to find the nuclear localization signal of NS1. The C-terminal deletion mutants of NS1 were expressed in COS-1 cells and the intracellular localization of the expressed mutants was analyzed by immunofluorescence. The wild type NS1 composed of residues 1-246 and C-terminal deletion mutant of residues 1-154 were localized in the cytoplasm predominantly. However, C-terminal deletion mutant of residues 1-108 was localized in the nucleus and cytoplasm. These results suggest that nuclear export signal of NS1 may be present between residues 109 and 154.
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Author(s)
Mizuta K, Abiko C, Aoki Y, Ikeda T, Matsuzaki Y, Hongo S, Itagaki T, Katsushima N, Ohmi A, Nishimura H, Ahiko T.
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Journal Title
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Pages: 400-5
DOI
Peer Reviewed
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DOI
Peer Reviewed
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