2013 Fiscal Year Final Research Report
Impact of hepatitis C virus infection in B cells on pathogenesis
| Project/Area Number |
23590547
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Kagoshima University |
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Principal Investigator |
KOHARA Kyoko 鹿児島大学, 獣医学部, 教授 (20225478)
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| Project Period (FY) |
2011 – 2013
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| Keywords | C型肝炎ウイルス / Bリンパ腫 / microarray / A20 / NF-kB / miR-26b |
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| Research Abstract |
Hepatitis C virus (HCV) infection leads to the development of hepatic diseases, as well as extra hepatic disorders such as B-cell non-Hodikin's lymphoma (B-NHL). To reveal the molecular signaling pathways responsible for HCV-associated B-NHL development, we utilized transgenic (Tg) mice that express the full-length HCV genome specifically in B cells and develop non-Hodgkin type B-cell lymphomas (BCLs). The gene expression profiles in B cells from BCL-developing HCV-Tg mice, from BCL-non-deceloping HCV-Tg mice, and from BCL-non-developing HCV-negative mice were analyzed by genome-wide microarray.
Markedly modified genes such as Fos, C3, LTbR, A20, NF-kB and miR-26b in BCLs were further characterized using specific assays. We propose that activation of both canonical and alternative NF-kB signaling pathways and down-regulation of miR-26b contribute to the development of HCV-associated B-NHL.
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[Journal Article] A serine palmytoiltransferase inhibitor inhibits hepatitis C virus replication in human hepatocytes2013
Author(s)
Katsume A, Tokunaga Y, Hirata Y, Munakata T, Saito M, Hayashi H, Okamoto Y, Kusanagi I, Fujiwara S, Klumpp K, Tsukiyama-Kohara K, El-Gohary A, Sudoh M, Kohara M
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Journal Title
Gastroenterology
Volume: 145(4)(pii : S0016-5085(13)00914-1. 10.1053)
Pages: 865-73
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[Journal Article] Targeted induction of interferon-λin humanized chimeric mouse liver abrogates hepatotropic virus infection2013
Author(s)
Nakagawa S, Hirata Y, Kameyama T, Tokunaga Y, Nishito Y, Hirabayashi K, Yano J, Ochiya T, Tateno C, Tanaka Y, Mizokami M, Tsukiyama-Kohara K, Inoue K, Yoshiba M, Takaoka A, Kohara M
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Journal Title
PLoS One
Volume: 8(3)
Pages: e59611
DOI
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[Journal Article] Immunization with a recombinant vaccinia virus that encodes nonstructural proteins of the hepatitis C virus suppr esses viral protein levels in mouse liver2012
Author(s)
Sekiguchi S, Kimura K, Chiyo T, Ohtsuki T, Tobita Y, Tokunaga Y, Yasui F, Tsukiyama-Kohara K, Wakita T, Tanaka T, Miyasaka M, Mizuno K, Hayashi Y, Hishima T, Matsushima K and Kohara M
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Journal Title
Pros One
Volume: 7(12)
Pages: e51656
DOI
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[Journal Article] Monoclonal antibody 2-152a suppresses hepatitis C virus infection thorough betaine/GABA transporter-12011
Author(s)
Satoh M, Saito M, Takano T, Kasama Y, Nishimura T, Nishito Y, Hirata Y, Arai M, Sudoh M, Kai C, Kohara M and Tsukiyama-Kohara K
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Journal Title
J Infect Dis
Volume: 204(8)
Pages: 1172-1180
DOI
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[Journal Article] Augmentation of DHCR24 expression by hepatitis C virus infection facilitates viral replication in hepatocytes2011
Author(s)
Takano T, Tsukiyama-Kohara K, Hayashi M, Hirata Y, Satoh M, Tateno C, Hayashi Y, Hishima T, Funata N, Sudo M, and Kohara M
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Journal Title
J Hepatology
Volume: 55(3)
Pages: 512-521
DOI
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