Evaluation of a minor sialic acid as an influenza A virus receptor base on functional glycomics approach.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590549
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: University of Shizuoka
• Principal Investigator: TAKAHASHI Tadanobu 静岡県立大学, 薬学部, 講師 (20405145)
• Co-Investigator(Kenkyū-buntansha): MINAMI Akira 静岡県立大学, 薬学部, 助教 (80438192)
• Project Period (FY): 2011 – 2013
• Keywords: 細胞 / シアル酸 / N-グリコリルノイラミン酸 / インフルエンザウイルス

Research Abstract

N-glycolylneuraminic acid (Neu5Gc) is said to be one of influenza A virus (IAV) receptors. However, expression of Neu5Gc on human cells suppressed infection of most Neu5Gc-binding human IAVs and equine IAVs. Neu5Gc is exogenously incorporated into human cells from Neu5Gc-rich foods such as red meat and milk. Neu5Gc on human cells may be involved in infection defense against many IAVs.

Research Products

• [Journal Article] Visualization of sialidase activity in mammalian tissues and cancer detection with a novel fluorescent sialidase substrate (2014)
  • Author(s): Akira Minami, Tadamune Otsubo, Daisuke Ieno, Kiyoshi Ikeda, Hiroaki Kanazawa, Kosuke Shimizu, Ko Ohata, Tsunehiro Yokochi, Yuuki Horii, Hokuto Fukumoto, Risa Taguchi, Tadanobu Takahashi, Naoto Oku, Takashi Suzuki
  • Journal Title: PLoS One Volume: 9 Pages: e81941
  • DOI: 10.1371/journal.pone.0081941
  • Peer Reviewed
• [Journal Article] Ferrier glycosylation reaction catalyzed by Bi(OTf)3-montmorillonite K-10: efficient synthesis of 3,4-unsaturated sialic acid derivatives : shynthesis and biological evaluation as inhibitors of human parainfluenza virus type 1 (2014)
  • Author(s): Mai Oba, Yayoi Ueno, Satoru Kitani, Takuya Hayakawa, Tadanobu Takahashi, Takashi Suzuki, Masayuki Sato, Kiyoshi Ikeda
  • Journal Title: Heterocycles Volume: 89 Pages: 89-81
  • Peer Reviewed
• [Journal Article] Sensitive and direct detection of receptor binding specificity of highly pathogenic avian influenza A virus in clinical samples (2013)
  • Author(s): Tadanobu Takahashi, Tatsuya Kawakami, Takashi Mizuno, Akira Minami, Yuko Uchida, Takehiko Saito, Shigeyuki Matsui, Makoto Ogata, Taichi Usui, Nongluk Sriwilaijaroen, Hiroaki Hiramatsu, Yasuo Suzuki, Takashi Suzuki
  • Journal Title: PLoS One Volume: 8 Pages: e78125
  • DOI: 10.1371/journal.pone.0081941
  • Peer Reviewed
• [Journal Article] Mutations in NA that induced low pH-stability and enhanced the replication of pandemic (H1N1) 2009 influenza A virus at an early stage of the pandemic (2013)
  • Author(s): Tadanobu Takahashi, Jiasheng Song, Takashi, Suzuki, Yoshihiro Kawaoka
  • Journal Title: PLoS One Volume: 8 Pages: e64439
  • Peer Reviewed
• [Journal Article] Binding kinetics of sulfatide with influenza A virus hemagglutinin (2013)
  • Author(s): Takahashi T, Kawagishi S, Masuda M, Suzuki T
  • Journal Title: Glycoconj. J. Volume: 30 Pages: 709-716
  • DOI: 10.1007/s10719-013-9477-7
  • Peer Reviewed
• [Journal Article] Sulfatide regulates caspase-3-independent apoptosis of influenza A virus through viral PB1-F2 protein (2013)
  • Author(s): Tadanobu Takahashi, Masahiro Takaguchi, Tatsuya Kawakami, Takashi Suzuki
  • Journal Title: PLoS One Volume: 8 Pages: e61092
  • DOI: 10.1371/journal.pone.0061092
  • Peer Reviewed
• [Journal Article] 2-(Benzothiazol-2-yl)-phenyl-β-D-galactop yranoside derivatives as fluorescent pigment dyeing substrates and their application for the assay ofβ-D-galactosidase activities (2013)
  • Author(s): Tadamune Otsubo, Akira Minami, Haruna Fujii, Risa Taguchi, Tadanobu Takahashi, Takashi Suzuki, Fumiteru Teraoka, Kiyoshi Ikeda
  • Journal Title: Bioorg. Med. Chem. Lett Volume: 23 Pages: 2245-2249
  • DOI: 10.1016/j.bmcl.2013.01.043
  • Peer Reviewed
• [Journal Article] Syntheses of 2-Deoxy-2,3-didehydro-N-acetylneuraminic Acid Analogues Modified byα-Acylaminoamido Groups at the C-4 Position Using Isocyanide-Based Four-Component Coupling and Biological Evaluation as Inhibitors of Human Parainfluenza Virus Type 1 (2013)
  • Author(s): Reiko Nishino, Takuya Hayakawa, Tadanobu Takahashi, Takashi Suzuki, Masayuki Sato, Kiyoshi Ikeda
  • Journal Title: Chem. Pharm. Bull Volume: 61 Pages: 69-74
  • DOI: 10.1248/cpb.c12-00834
  • Peer Reviewed
• [Journal Article] Amino acid determinants conferring stable sialidase activity at low pH for H5N1 influenza A virus neuraminidase (2012)
  • Author(s): Tadanobu Takahashi, Chairul A. Nidom, Mai thi QuynhLe, Takashi Suzuki, Yoshihiro Kawaoka
  • Journal Title: FEBS Open Bio Volume: 2 Pages: 261-266
  • DOI: 10.1016/j.fob.2012.08.007
  • Peer Reviewed
• [Journal Article] Sulfatide Negatively Regulates the Fusion Process of Human Parainfluenza Virus Type 3 (2012)
  • Author(s): Tadanobu Takahashi, Kazuhiko Ito, Keijo Fukushima, Masahiro Takaguchi, Takuya Hayakawa, Yasuo Suzuki, Takashi Suzuki
  • Journal Title: J. Biochem Volume: 152 Pages: 373-380
  • DOI: 10.1093/jb/mvs080
  • Peer Reviewed
• [Journal Article] Comparative Analyses of N-Glycosylation Profiles of Influenza A Viruses Grown in Different Host Cells (2012)
  • Author(s): Hirokazu Yagi, Shinya Watanabe, Takashi Suzuki, Tadanobu Takahashi, Yasuo Suzuki, Koichi Kato
  • Journal Title: Open Glycoscience Volume: 5 Pages: 2-12
  • Peer Reviewed
• [Journal Article] Plaque formation assay for human parainfluenza virus type 1 (2011)
  • Author(s): Keijo Fukushima, Tadanobu Takahashi, Masahiro Takaguchi, Hiroo Ueyama, Seigo Ito, Yuuki Kurebayashi, Tomohiro Kawanishi, Jennifer L. Mckimm-Breschkin, Toru Takimoto, Akira Minami, Takashi Suzuki
  • Journal Title: Biol. Pharm. Bull Volume: 34 Pages: 996-1000
  • DOI: 10.1248/bpb.34.996
  • Peer Reviewed
• [Journal Article] A single amino acid mutation at position 170 of human parainfluenza virus type 1 fusion glycoprotein induces obvious syncytium formation and caspase-3-dependent cell death (2011)
  • Author(s): Masahiro Takaguchi, Tadanobu Takahashi, Chika Hosokawa, Hiroo Ueyama, Keijo Fukushima, Takuya Hayakawa, Kazuhiko Itoh, Kiyoshi Ikeda, Takashi Suzuki
  • Journal Title: J. Biochem Volume: 149 Pages: 191-202
  • DOI: 10.1093/jb/mvq139
  • Peer Reviewed
• [Journal Article] Syntheses of 2-deoxy-2,3-didehydro-N-acetylneuraminic acid analogues modified by N-sulfonylamidino groups at the C-4 position and biological evaluation as inhibitors of human parainfluenza virus type 1 (2011)
  • Author(s): Reiko Nishino, Kiyoshi Ikeda, Takuya Hayakawa, Tadanobu Takahashi, Takashi Suzuki, Masayuki Sato
  • Journal Title: Bioorg. Med. Chem Volume: 19 Pages: 2418-2427
  • DOI: 10.1016/j.bmc.2011.02.010
  • Peer Reviewed
• [Presentation] ウイルス感染における糖鎖の機能解明 (2013)
  • Author(s): 高橋忠伸
  • Organizer: 第59回日本薬学会東海支部総会・大会
  • Place of Presentation: 名古屋
  • Year and Date: 2013-07-06
  • Invited
• [Presentation] インフルエンザウイルスの分泌型ヘマグルチニンの精製とスルファチド結合パラメータの測定 (2012)
  • Author(s): 高橋忠伸、川岸佐和子、鈴木隆
  • Organizer: 第6回QCM研究会
  • Place of Presentation: 東京
  • Year and Date: 2012-08-23
  • Invited
• [Presentation] Sulfatide negatively regulates the fusion process of human parainfluenza virus type 3 (2012)
  • Author(s): Tadanobu Takahashi, Kazuhiko Ito, Keijo Fukushima, Masahiro Takaguchi, Takuya Hayakawa, Yasuo Suzuki, Takashi Suzuki
  • Organizer: 26th International Carbohydrate Symposium
  • Place of Presentation: Madrid, Spain)
  • Year and Date: 2012-07-26
• [Presentation] インフルエンザウイルスおよびヒトパラインフルエンザウイルス感染におけるスルファチドの役割 (2011)
  • Author(s): 高橋忠伸
  • Organizer: 日本農芸化学会中部支部第163回例会若手シンポジウム
  • Place of Presentation: 静岡
  • Year and Date: 2011-12-03
  • Invited
• [Presentation] A single amino acid mutation at position 170 of human parainfluenza virus type 1 fusion glycoprotein induces obvious syncytium formation and caspase-3-dependent cell death (2011)
  • Author(s): Tadanobu Takahashi, Masahiro Takaguchi, Chika Hosokawa, Hiroo Ueyama1, Keijo Fukushima, Takuya Hayakawa, Kazuhiko Itoh, Kiyoshi Ikeda and Takashi Suzuki
  • Organizer: International Union of Microbiological Societies 2011 Congress (IUMS2011)
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-13
• [Book] 薬事日報, 糖鎖生物学を背景にしたウイルス研究, 研究戦略YAKU学-研究現場から臨床へ- (2013)
  • Author(s): 高橋忠伸
  • Total Pages: 8
  • Publisher: 薬事日報社
• [Remarks] 静岡県立大学 薬学部 生化学分野HP
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/biochem/index.html
• [Remarks] 静岡県立大学 薬学部 大学院薬学研究院HP
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/
• [Remarks] 静岡県立大学HP
  • URL: http://www.u-shizuoka-ken.ac.jp/