2013 Fiscal Year Final Research Report
Role of zinc trensporter in allergic reaction
| Project/Area Number |
23590576
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
NISHIDA KEIGO 独立行政法人理化学研究所, 統合生命医科学研究センター, 上級研究員 (80360618)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 亜鉛トランスポーター / マスト細胞 / 好塩基球 / サイトカイン / アレルギー / 腹膜炎 |
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| Research Abstract |
Zinc (Zn) is an essential nutrient and its deficiency causes immunodeficiency. Electron microscopic studies demonstrated that mast cell granules are rich in Zn. However, the precise roles and molecular mechanism(s) of Zn`s function in mast cells have not been clarified. It has been considered that Zn is supplied to granule by Zn transporter. We identified that Zn transporter ZnT2/Slc30a2 was localized at granule membrane in mast cell. To understand role of granule`s Zn in mast cell, we generated ZnT2-KO mice. We found that survival rate in cecal ligation and puncture was diminished in ZnT2-KO mice.
It has reported Zn is required for activity of CPA3 (carboxypeptidase3) in mast cell and loss of CPA3 activity causes endothelin-induced sepsis shock. Consistent with this report, CPA3 activity was decreased in ZnT2-KO mast cell. In this study, we showed that ZnT2 is important for survival from sepsis and required for Zn-dependent CPA3 activity.
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[Journal Article] Signal-transducing adaptor protein-2 controls the IgE-mediated, mast cell-mediated anaphylactic responses2014
Author(s)
Sekine Y., Nishida K, Yamasaki S, Muromoto R, Kon S, Kashiwakura J, Saitoh K, Togi S, Yoshimura A, Oritani K, and Matsuda T
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Journal Title
J Immunol
Volume: 192
Pages: 3488-3495
DOI
Peer Reviewed
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[Journal Article] Vaccination with antigen mRNA-transfected, NKT cell ligand-loaded, human vector cells elicits robust in situ immune responses by dendritic cells2012
Author(s)
Shimizu K., Mizuno T, Shinga J, Asakura M, Kakimi K, Ishii Y, Masuda K, Maeda T, Sugahara H, Sato Y, Matsushita H, Nishida K, Hanada K, Dorrie J, Schaft N, Bickham K, Koike H, Ando T, Nagai R and Fujii S
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Journal Title
Cancer Res
Volume: 73
Pages: 62-73
DOI
Peer Reviewed
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[Journal Article] Endothelial Gab1 Deletion Accelerates Angiotensin II-Dependent Vascular Inflammation and Atherosclerosis in Apolipoprotein E Knockout Mice2012
Author(s)
Higuchi K., Nakaoka Y, Shioyama W, Arita Y, Hashimoto T, Yasui T, Ikeoka K, Kuroda T, Minami T, Nishida K, Fujio Y, Yamauchi-Takihara K, Shirai M, Mochizuki N, Komuro I
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Journal Title
Circulation Journal
Volume: 76
Pages: 2031-2040
DOI
Peer Reviewed
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[Journal Article] Crucial role for autophagy in degranulation of mast cells2011
Author(s)
Ushio H, Ueno T, Kojima Y, Komatsu M, Tanaka S, Yamamoto A, Ichimura Y, Ezaki J, Nishida K, Komazawa-Sakon S, Niyonsaba F, Ishii T, Yanagawa T, Kominami E, Ogawa H, Okumura K, Nakano H
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Journal Title
J Allergy Clin Immunol
Volume: 127
Pages: 1267-1276
DOI
Peer Reviewed
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