Development of long-term culture system for human MDS specific iPS

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590660
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Gunma University
• Principal Investigator: HANDA HIROSHI 群馬大学, 医学部附属病院, 講師 (90282409)
• Co-Investigator(Kenkyū-buntansha): MURAKAMI Hirokazu 群馬大学, 大学院保健学研究科, 教授 (40166260)
• Project Period (FY): 2011 – 2013
• Keywords: iPS細胞 / 骨髄異形成症候群 / 細胞外マトリックス / 長期培養

Research Abstract

Myelodysplastic syndrome (MDS) is the disease characterized by peripheral blood cytopenia, morphological abnormality of hematopoietic cells and chromosomal abnormality. MDS is also a lethal disease having two sides; bone marrow failure and pre-leukemia. In order to develop in vitro model system for MDS, we performed basic study to develop long term culture system of the disease specific iPS cell using cell free with extra-cellular matrix. Bone marrow specimens obtained from MDS patients after informed consent were separated and CD34 positive hematopoietic progenitor fraction was purified. Five transcription factors; OCT-4, SOX-2, KLF-4, LIN28, NANOG, were transfected to the purified hematopoietic progenitor cells to produce MDS-iPS, however we failed to maintain, grow and differentiate this iPS cells.