2013 Fiscal Year Final Research Report
Analysis of phospholipid metabolism in interstitial pneumonia and development of a laboratory assay using mass spectrometry
| Project/Area Number |
23590665
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Takayuki 信州大学, 医学部, 教授 (80238815)
HONGO Minoru 信州大学, 医学部, 教授 (40209317)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 臨床化学 / 脂質 / 質量分析 |
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| Research Abstract |
Bronchoalveolar lavage fluid (BALF) is used for diagnosis and determining clinical condition in interstitial pneumonia, but the relations between BAL lipid composition and clinical condition are unclear. We developed an assay for determining lipid species, fatty acid composition, and lipid metabolic enzyme activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and gas chromatography mass spectrometry. The methods were rapid, simple, and accurate with very small samples. The BALF phospholipid profile was determined by mass spectrometry. We could detect changes in metabolism of phospholipid species in BALF by comparing the phospholipid profile of the serum with BAL. The results may be useful as an index to determined phospholipid metabolic disorders in pulmonary surfactant and alveolar cells.
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[Journal Article] High concentrations of omega-3 fatty acids are associated with the development of atrial fibril- lation in the Japanese population2013
Author(s)
Tomita T, Hata T, Takeuchi T, Oguchi Y, Okada A, Aizawa K, Koshikawa M, Ota giri K, Motoki H, Kasai H, Izawa A, Koyama J, Hongo M, Ikeda U
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Journal Title
Heart Vessels
Volume: 28(4)
Pages: 497-504
Peer Reviewed
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