Establishment of quick detection system of chikungunya-infected mosquitoes for preventing from an outbreak of the secondary-infected patients

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590803
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Public health/Health science
• Research Institution: Oita University
• Principal Investigator: ESHITA Yuki 大分大学, 医学部, 准教授 (10082223)
• Project Period (FY): 2011 – 2013
• Keywords: 媒介蚊対策 / 迅速遺伝子診断 / チクングニア熱 / 蚊 / RT-LAMP / チクングニアウイルス / 感受性 / リスク評価

Research Abstract

The susceptibility and risk evaluation of Japanese mosquitoes to chikungunya virus, and introduction of RT-LAMP method were evaluated in experimental conditions. It was revealed that the three mosquito species, Aedes albopictus, Ae. flavopictus, and Aedes riversi had transmission ability for the virus. In addition, the RT-LAMP method was superior to the RT-PCR method as the quick pathogen detection system. Aedes albopictus was estimated as the mosquito, which was the highest in a risk of the pathogen spread in Japan. Pathogen detection system including these results may contribute to prevent from an outbreak of the secondary-infected patients.

Research Products

• [Journal Article] Vector competence of Aedes aegypti to dengue virus (2014)
  • Author(s): Lucky Ronald Runtuwene, Kaori Noguchi, Akinori Tokunaga, Takashi Kobayashi, Kenta Nakai and Yuki Eshita
  • Journal Title: Urban Pest Management Volume: 4(1)(印刷中)
  • Peer Reviewed
• [Journal Article] Dengue transmission model by means of viremic adult immuno-competent mouse (2014)
  • Author(s): Lucky Ronald Runtuwene, Eiji Konishi, Atsushi Yamanaka, Yoshihiro Makino, Yutaka Suzuki, Tomohiko Takasaki, Ichiro Kurane, Takashi Kobayashi and Yuki Eshita
  • Journal Title: Parasites & Vectors Volume: 7 Pages: 143
  • DOI: 10.1186/1756-3305-7-143
  • Peer Reviewed
• [Journal Article] Inhibitory effects of JNK on Aedes albopictus early larval development (2012)
  • Author(s): Tomomitsu Satho, Yuki Nagano, Yuki Eshita, Yujin Hisatomi, Akira Sakata, Takeshi Miyata, Nobuhiro Kashige, Fumio Miake, Lucky R Runtuwene, Shuetsu Fukushi, Masayuki Saijyo, Ichiro Kurane, Shigeru Morikawa and Tetsuya Mizutani
  • Journal Title: Urban pest management Volume: 2(1) Pages: 7-13
  • Peer Reviewed
• [Journal Article] Field-collected permethrin-resistant Aedes aegypti from central Thailand contain point mutations in the domain IIS6 of the sodium channel gene (KDR). Southeast Asian J. Trop. Med (2012)
  • Author(s): Raweewan Srisawat, Narumon Komalamisra, Chamnarn Apiwathnasorn, Pungasem Paeporn, Sittiruk Roytrakul, Yupha Rongsriyam, Yuki Eshita
  • Journal Title: Public Health Volume: 43(6) Pages: 1380-1386
  • Peer Reviewed
• [Journal Article] Prevalence of Phasi Charoen virus in female mosquitoes (2011)
  • Author(s): Yusuke Sayama, Yuki Eshita, Takuya Yamao, Miho Nishimura, Tomomitsu Satho, Raweewan Srisawat, Narumon Komalamisra, Yupha Rongsriyam, Kouji Sakai, Shuetsu Fukushi, Masayuki Saijo, Hitoshi Oshitani, Ichiro Kurane, Shigeru Morikawa and Tetsuya Mizutani
  • Journal Title: Journal of Parasitology and Vector Biology Volume: 3(1) Pages: 19-21
  • Peer Reviewed
• [Journal Article] Present status of the insecticide susceptibility of Aedes mosquitoes in Thailand (2011)
  • Author(s): Raweewan Srisawat, Narumon Komalamisra, Theerawit Phanphoowong, Tomohiko Takasaki, Lucky Ronald Runtuwene, Ichiro Kurane, Hironari Narita, Yuki Eshita
  • Journal Title: Journal of Japanese Red Cross Toyota College of Nursing Volume: 6(1) Pages: 31-37
  • Peer Reviewed
• [Presentation] 本邦産Aedes (Stegomyia) scutellarisグループ蚊2種のチクングニアウイルス感受性(2) (2014)
  • Author(s): 江下優樹, 福田昌子, Lucky Runtuwene, 大塚靖, 野口香緒里, 川上絵理, 德永暁憲, 小林隆志, 服部正策, Raweewan Srisawat, Narumon Komalamisra, 牛島廣治, 倉根一郎, 高崎智彦
  • Organizer: 第66回日本衛生動物学会大会
  • Place of Presentation: 岐阜大学地域科学部、岐阜県岐阜市(Med. Entomol. Zool., 65 (大会特集号) :45, 2014)
  • Year and Date: 20140321-23
• [Presentation] 本邦産Aedes (Stegomyia) scutellarisグループ蚊2種のチクングニアウイルス感受性 (2013)
  • Author(s): 江下優樹, 福田昌子, Lucky Runtuwene, 大塚靖, 野口香緒里, 川上絵理, 德永暁憲, 小林隆志, 服部正策, Raweewan Srisawat, Narumon Komalamisra, 牛島廣治, 倉根一郎, 高崎智彦
  • Organizer: 第66回日本寄生虫学会南日本支部大会・第63回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 大分県由布市、大分大学医学部臨床講義棟1F臨床中講義室(第66回日本寄生虫学会・第63回日本衛生動物学会南日本支部合同大会プログラム講演要旨:9, 2013. Med. Entomol. Zool., 64(2), 2013)
  • Year and Date: 20131102-03
• [Presentation] INOVATIVE TECHNOLOGY FOR USE IN VECTOR CONTROL (2013)
  • Author(s): Yuki Eshita, Lucky R. Runtuwene, Sachie Matsubara, Kaori Noguchi, Eri Kawakami, Masako Fukuda, Yasushi Otsuka, Akinori Tokunaga, Takashi Kobayashi, Raweewan Srisawat, Narumon Komalamisra, Yupha Rongsriyam, Bouasy Hongvanthong, Arthur E. Mongan, Josef Tuda, Mihoko Imada, Shuichi Kawashima, Junya Yamagish, Yutaka Suzuki, Sumio Sugano, Kenta Nakai, Ryuichiro Maeda, Chihiro Sugimoto, Hironari Narita, Hiroshi Ushijima, Koichi Morita, Tomohiko Takasaki, Ichiro Kurane
  • Organizer: The 1^<st> conference on asian pediatric infectious diseases(第1回アジア小児感染症会議)
  • Place of Presentation: 東京大学医学部3号館N101室、東京都文京区
  • Year and Date: 20130824-26
• [Presentation] 節足動物媒介性ウイルスの迅速検出へのRT-LAMP法の改良 (2013)
  • Author(s): 江下優樹, 松原祥恵, Lucky R.Runtuwene, 野口香緒里, 川上絵理, 大塚靖, 福田昌子, 小林隆志, 高崎智彦, Raweewan Srisawat, Narumon Komalamisra, Bouasy Hongvanthong, 成田弘成, 牛島廣治, Arthur E. Mongan, 今田美穂子, 前田龍一郎, 森田公一, 杉本千尋, 倉根一郎
  • Organizer: 日本家屋害虫学会第34回大会・総会
  • Place of Presentation: 日本大学生物資源科学部、神奈川県藤沢市
  • Year and Date: 20130622-23
• [Presentation] New emerging technology for use in vector control (2012)
  • Author(s): Yuki Eshita, Lucky R. Runtuwene, Raweewan Srisawat, Narumon Komalamisra, Yupha Rongsriyam, Shuichi Kawashima, Junya Yamagishi, Yutaka Suzuki, Sumio Sugano, Kenta Nakai, Ryuichiro Maeda, Chihiro Sugimoto, Tomohiko Takasak, Ichiro Kurane
  • Organizer: Joint International Tropical Medicine Meeting 2012 (JITMM 2012) and The 7th Seminar on Food- and Water-Borne Parasitic Zoonoses (FBPZ7), Symposium session on S34 Entomological approaches for the study of arboviruses
  • Place of Presentation: Centara Grand & Bangkok Convention Centre At Central World, Bangkok, Thailand(Abstract of Joint International Tropical Medicine Meeting 2012 (JITMM 2012))
  • Year and Date: 20121212-14
• [Presentation] リバ-ズシマカの系統確立および奄美大島・鹿児島県佐多岬でのその生息環境 第65回日本寄生虫学会南日本支部大会・第62回日本衛生動物学会南日本支部大会合同大会 (2012)
  • Author(s): 江下優樹, Lucky R. Runtuwene, 大塚靖, 松原祥恵, 小林隆志, 川島秀一, 服部正策, 倉石武, 甲斐智恵子, Raweewan Srisawat, Narumon Komalamisra, Yupha Rongsriyam, Arthur E. Mongan, 前田龍一郎, 杉本千尋, 牛島廣治, 高崎智彦, 倉根一郎
  • Organizer: 第65回日本寄生虫学会・第62回日本衛生動物学会南日本支部合同大会
  • Place of Presentation: 長崎県長崎市、長崎大学医学部ポンペ会館(第65回日本寄生虫学会・第62回日本衛生動物学会南日本支部合同大会プログラム講演要旨, 2012. Med. Entomol. Zool., 64(2), 2013)
  • Year and Date: 20121110-11
• [Presentation] RT-LAMP法を用いた蚊からのアルボウイルスゲノムの迅速検出 (2012)
  • Author(s): 江下優樹, Lucky R. Runtuwene, 高崎智, Raweewan Srisawat, Narumon Komalamisra, Yupha Rongsriyam, Bouasy Hongvanthong, 牧野芳大, 成田弘成, 牛島廣治, Arthur E. Mongan, 今田美穂子, 前田龍一郎, 森田公一, 杉本千尋, 倉根一郎
  • Organizer: 第64回日本衛生動物学会大会
  • Place of Presentation: 信州大学、長野県上田市(Med. Entomol. Zool., 63 (大会特集号) :64, 2012)
  • Year and Date: 20120330-31
• [Presentation] Novel method using mice to infect Aedes aegypti with dengue virus type 2 (2011)
  • Author(s): Lucky R. Runtuwene, Atsushi Yamanaka, Eiji Konishi, Yoshihiro Makino, Yutaka Suzuki, Tomohiko Takasaki, Ichiro Kurane and Yuki Eshita
  • Organizer: 第63回日本衛生動物学会大会
  • Place of Presentation: 国立感染症研究所、一橋記念講堂東京都(Med. Entomol. Zool., 62 (大会特集号) :74, 2011)
  • Year and Date: 20110414-16
• [Presentation] RT-LAMP法を用いた蚊からのデングウイルスゲノムの迅速検出 (2011)
  • Author(s): 江下優樹, Lucky R. Runtuwene,高崎智彦, Raweewan Srisawat, Narumon Komalamisra, Yupha Rongsriyam, Bouasy Hongvanthong, Boualy Kheokhamphavanh, Bounpone Sidavong, Kham Thong, Silivanh Chanthalavong, Khambang Silavong, Kalounna Keokenechanh, Hongkham Keomanila, 牧野芳大, 成田弘成, 牛島廣治, Arthur E. Mongan, 前田龍一郎, 森田公一, 杉本千尋, 倉根一郎
  • Organizer: 第63回日本衛生動物学会大会
  • Place of Presentation: 国立感染症研究所、一橋記念講堂東京都(Med. Entomol. Zool.,62(大会特集号):75, 2011)
  • Year and Date: 20110414-16
• [Presentation] 当科で経験したデング熱の一症例 (2011)
  • Author(s): 鳥羽聡史, 甲斐直子, 阿南栄一朗, 岡宏亮, 横山敦, 大谷哲史, 石井寛, 岸建志, 白井亮, 時松一成, 平松和史, 山田健太郎, アハメド・カムルディン, 江下優樹, 西園晃, 門田淳一
  • Organizer: 大分感染症研究会第48回例会
  • Place of Presentation: 大分東洋ホテル,大分市(大分感染症研究会第48回例会プログラム)
  • Year and Date: 2011-03-24
• [Presentation] デング熱の1例
  • Author(s): 野柳俊明, 江下優樹
  • Place of Presentation: (西日本皮膚科74(6):655, 2012)