Study of the mechanizum of acute encephalopathy caused by cyanogenic mushroom

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590848
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: GONMORI Kunio 浜松医科大学, 医学部, 特任研究員 (10006744)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Osamu 浜松医科大学, 医学部, 理事 (70093044) ; WATANABE Kanako 浜松医科大学, 医学部, 教授 (70288546)
• Project Period (FY): 2011 – 2013
• Keywords: きのこ中毒 / 急性脳症 / 青酸 / スギヒラタケ / 培養

Research Abstract

In autumn 2004, 59 cases of acute encephalopathy were reported across 9 prefectures in Japan.We concluded that encephalopathy experienced caused by was the cyanogenic mushroom.We study the mechanism of the acute encephalopathy by cyanogenic mushroom, Pleurocybella porrigens. For three years from 2011, we collect the mushrooms at a same point.But the occureence of mushroom is not enough except 2012 by unseasonable weather.It is difficult to study with natural materials is affected by the environment such as weather. We tried to artificial cultivation the Pleurocybella porrigens.

Research Products

• [Journal Article] Analysis of ibotenic acid and muscimol in Amanita mushrooms by hydrophilic interaction liquid chromatography-tandem mass spectrometry (2012)
  • Author(s): Gonmori K, Hasegawa K, Fujita H, Kamijo Y, Nozawa H, Yamagishi I, Minakata K,Watanabe K, Suzuki O
  • Journal Title: Forensic Toxicology Volume: 30(2) Pages: 168-172
• [Journal Article] MALDI-TOF mass spectrometric analysis ofα-amanitin,β-amanitin, and phalloidin in urine (2012)
  • Author(s): Gonmori K, Minakata K, Suzuki M, Yamagishi I, Nozawa H, Hasegawa K, Wurita A,Watanabe K, Suzuki O
  • Journal Title: Forensic Toxicology Volume: 30(2) Pages: 179-184
• [Journal Article] A new approach in tandem mass spectrometric simultaneous determination of perchlorate, thiocyanate, and iodide using their ternary complexes with tris(1,10-phenanthroline)iron(II) chelate (2012)
  • Author(s): Minakata K, Nozawa H, Yamagishi I, Gonmori K, Hasegawa K, Suzuki M, Wurita A, Watanabe K, Suzuki O
  • Journal Title: Forensic Toxicology Volume: 30(2) Pages: 156-161
• [Journal Article] Mushroom toxins : a forensic toxicological review (2011)
  • Author(s): Gonmori K, Fujita H, Yokoyama K, Watanabe K, Suzuki O
  • Journal Title: Forensic Toxicol Volume: 29 Pages: 85-94
• [Journal Article] Determination of cyanide in blood by electrospray ionization tandem mass spectrometry after direct injection of dicyanogold (2011)
  • Author(s): Minakata K, Nozawa H, Gonmori K, Yamagishi I, Suzuki M, Hasegawa K, Watanabe K, Suzuki O
  • Journal Title: Anal Bioanal Chem Volume: 400 Pages: 1945-1951
• [Presentation] MALDI-Q-TOF mass spectrometric determination of alpha-amanitin, beta-amanitin, and phalloidin in urine (2013)
  • Author(s): Gonmori K, Minakata K, Suzuki M, Hasegawa K, Yamagishi I, Nozawa H, Wurita A, Watanabe K, Suzuki O
  • Organizer: The 51th Annual Meeting of the International Association of Forensic Toxicologists 2013
  • Place of Presentation: Funchal, Madeira, Portugal
  • Year and Date: 20130000
• [Presentation] Determination of azide in gastric fluid and urine by flow-injection electrospray ionization tandem mass spectrometry (2012)
  • Author(s): Minakata K, Gonmori K, Nozawa H, Yamagishi I, Hasegawa K, Wurita A, Watanabe K, Suzuki O
  • Organizer: The 50th Annual Meeting of the International Association of Forensic Toxicologists 2012
  • Place of Presentation: Hamamatsu, Japan
  • Year and Date: 20120000
• [Presentation] A sensitive and fully automated method for GC-MS analysis of volatile organic compounds in whole blood using large volumes of headspace vapor (2012)
  • Author(s): Hasegawa K, Wurita A, Gonmori K, Nozawa H, Yamagishi I, Minakata K, Suzuki O, Watanabe K
  • Organizer: The 50th Annual Meeting of the International Association of Forensic Toxicologists 2012
  • Place of Presentation: Hamamatsu, Japan
  • Year and Date: 20120000
• [Presentation] Analysis of ibotenic acid and muscimol in Amanita mushrooms by hydrophilic interaction LC-MS-MS with solid-phase extraction (2012)
  • Author(s): Gonmori K, Hasegawa K, Fujita H, Kmijo Y, Nozawa H, Yamagishi I, Minakata K, Watanabe K, Suzuki O
  • Organizer: The 50th Annual Meeting of the International Association of Forensic Toxicologists 2012
  • Place of Presentation: Hamamatsu, Japan
  • Year and Date: 20120000