2013 Fiscal Year Final Research Report
Development of an in vitro model system for drug metabolism studies with human hepatocyte
| Project/Area Number |
23590866
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | National Research Institute of Police Science |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 乱用薬物 / 代謝 / 肝細胞 / スフェロイド |
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| Research Abstract |
The usefulness of a hepatocyte spheroid array kit that enables simple three-dimensional hepatocyte culture as a tool to predict the metabolic fate of designer drugs was evaluated by comparing the metabolic pattern of designer drugs in hepatocyte culture to that of in vivo studies. The in vivo main metabolites of the designer drugs tested were also detected in the culture medium of the hepatocyte spheroids, indicating that the in vivo metabolic patterns of designer drugs were successfully reproduced using the hepatocyte spheroid array kit. The in vitro system developed in this study was found to be an effective tool for predicting the metabolic fate of designer drugs.
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