Analysis of immune and metabolic responses of liver cells under inflammatory circumstances or viral infection

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590970
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nagoya University
• Principal Investigator: ISHIKAWA TETSUYA 名古屋大学, 医学(系)研究科(研究院), 教授 (10288508)
• Co-Investigator(Kenkyū-buntansha): ISHIGAMI Masatoshi 名古屋大学, 医学部附属病院, 講師 (90378042) ; UEYAMA Jun 名古屋大学, 大学院医学系研究科, 准教授 (00397465)
• Project Period (FY): 2011 – 2013
• Keywords: 肝細胞 / サイトカイン / ケモカイン / 炎症応答

Research Abstract

To elucidate the role of hepatic parencymal cells on the pathogenesis of hepatitis, the expression patterns of cytokines and chemokines by hepatoma cell lines under inflammatory circumstance were analyzed. In three hepatoma cell lines used, the expressions of inflammatory cytokines and chemokies were up-regulated after various inflammatory stimuli. The pathogenetic importance of hepatic parencymal cells in the formation of hepatic inflammation is confirmed, and in vitro hepatitis model used in the current study is thought to be useful for the detailed analysis of the pathogenesis of hepatitis.

Research Products

• [Journal Article] Ursodeoxycholic acid inhibits overexpression of P-glycoprotein induced bydoxorubicininHepG2 cells (2014)
  • Author(s): Komori Y, Arisawa A, Takai M, Yokoyama K, Honda M, Hayashi K, Ishigami M, Katano Y, Goto H, Ueyama J, Ishikawa T, Wakusawa Y
  • Journal Title: Eur J Pharmacol Volume: 724 Pages: 161–167
  • DOI: 10.1016/j.ejphar.2013.12.023
  • Peer Reviewed
• [Journal Article] A pediatric case of hepatitis B virus subgenotype A2 in Japan (2013)
  • Author(s): Hayashi K, Ishigami M, Ishizu Y, Kuzuya T, Honda T, Itoh A, Hirooka Y, Ishikawa T, Nakano I, Ito Y, Kimura H, Katano Y, Goto H
  • Journal Title: Clin J Gastroenterol Volume: 6 Pages: 383–385
  • DOI: 10.1007/s12328-013-0402-1
  • Peer Reviewed
• [Journal Article] Pegylated interferon monotherapy in patients with chronic hepatitis C with low viremia and its relationship to mutations in the NS5A region and the single nucleotide polymorphism of interleukin-28B (2013)
  • Author(s): Hayashi K, Katano Y, Masuda H, Ishizu I, Kuzuya T, Honda T, Ishigami M, Itoh A, Hirooka Y, Nakano I, Ishikawa T, Urano F, Yoshioka K, Toyoda H, Kumada T, Goto H
  • Journal Title: Hepatol Res Volume: 43 Pages: 580–588
  • DOI: 10.1111/hepr.12005
  • Peer Reviewed
• [Journal Article] Comparison of the efficacy of ribavirin plus peginterferon alfa-2b for chronic hepatitis C infection in patients with and without coagulation disorders (2013)
  • Author(s): Honda T, Katano Y, Kuzuya T, Hayashi K Ishigami M, Itoh A, Hirooka Y, Nakano I, Ishikawa T, Toyoda H, Kumada T, Yamamoto K, Matsushita T, Kojima T, Takamatsu J, Goto H
  • Journal Title: J Med Virol Volume: 85 Pages: 228–234
  • DOI: 10.1002/jmv.23444
  • Peer Reviewed
• [Journal Article] Immunoregulation of hepatitis B virus infection -rationale and clinical application- (2012)
  • Author(s): Ishikawa T
  • Journal Title: Nagoya J Med Sci Volume: 74 Pages: 217-232
• [Journal Article] Prevalence of hepatitis C virus genotype 1a in Japan and correlation of mutations in the NS5A region and single-nucleotide polymorphism of interleukin-28B with the response to combination therapy with pegylated-interferon-alpha 2b and ribavirin (2012)
  • Author(s): Hayashi K, Katano Y, Kuzuya T, Tachi Y, Honda T, Ishigami M, Itoh A, Hirooka Y, Ishikawa T, Nakano I, Urano F, Yoshioka K, Toyoda H, Kumada T, Goto H
  • Journal Title: J Med Virol Volume: 84 Pages: 438-444
  • Peer Reviewed
• [Journal Article] Association of interleukin 28B and mutation in the core and NS5A region of hepatitis C virus with response to peg-interferon and ribavirin therapy (2011)
  • Author(s): Hayashi K, Katano Y, Honda T, Ishigami M, Itoh A, Hirooka Y, Ishikawa T, Nakano I, Yoshioka Y, Toyoda H, Kumada T, Goto H
  • Journal Title: Liver Int Volume: 31 Pages: 1359-1361
  • Peer Reviewed
• [Presentation] インドールアミン酸素添加酵素の発現抑制を用いたHBV特異的細胞障害性T細胞誘導効果の検討 (2013)
  • Author(s): 伊藤弘康、安藤達也、石川哲也、清島満
  • Organizer: 第60回日本臨床検査医学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20131031-1103
• [Presentation] ヒト肝癌細胞株間での各種炎症刺激に対するケモカイン産生能の比較 (2013)
  • Author(s): 山田達也、中川伸吾、吉住寧真、加納由貴、加納綾乃、石川哲也
  • Organizer: 第60回日本臨床検査医学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20131031-1103
• [Presentation] グレリン、レプチンによる炎症応答制御について (2013)
  • Author(s): 加納由貴、中川伸吾、吉住寧真、加納綾乃、山田達也、松浦俊博、石川哲也
  • Organizer: 第60回日本臨床検査医学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20131031-1103
• [Presentation] マウスB型急性肝炎モデルにおけるインドールアミン酸素添加酵素の解析 (2013)
  • Author(s): 伊藤弘康、大瀧博文、安藤達也、安藤量基、石川哲也、森脇久隆、清島満
  • Organizer: 第49回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 20130606-07