2013 Fiscal Year Final Research Report
Identification of microRNAs involved in the development of diabetic nephropathy
| Project/Area Number |
23591177
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Kidney internal medicine
|
|---|
| Research Institution | Akita University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMADA Yuichiro 秋田大学, 大学院医学系研究科, 教授 (60283610)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 糖尿病性腎症 / マイクロRNA |
|---|
| Research Abstract |
MicroRNAs (miRs) are a family of non-coding RNAs and inhibit target gene expression by causing messenger RNA degradation or by blocking protein translation. We analysed the specific expression of miRs by microarray and real-time RT-PCR using RNA extracted from whole kidneys and isolated glomeruli in two Akita diabetic mouse models which have different susceptibility to diabetic nephropathy, nephropathy-resistant C57BL/6-Akita and nephropathy-prone KK/Ta-Akita. Several miRs whose expression levels are different between C57BL/6-Akita and KK/Ta-Akita were identified in both whole kidney and glomerulus. These results indicate that the identified miRs may play an important role in the development and progression of diabetic nephropathy.
|
