2013 Fiscal Year Final Research Report
Molecular Mechanism for Phosphate Sensing and Bone-Kidney Functional Interaction
| Project/Area Number |
23591227
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Research Institute, Osaka Medical Center for Maternal and Child Health |
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Principal Investigator |
MICHIGAMI Toshimi 地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), 環境影響部門, 部長 (00301804)
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| Project Period (FY) |
2011 – 2013
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| Keywords | リン代謝 / 骨細胞 / FGF23 / DMP1 / PHEX / ビタミンD |
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| Research Abstract |
Recent studies suggest the roles of osteocyte in phosphate metabolism, although the underlying mechanisms remain unclear. In the current study, the roles of osteocytes in phosphate metabolism were investigated using the osteoblasts and osteocytes isolated from mouse long bones. The expression of Fgf23, Dmp1, Phex and Fam20c was higher in osteocytes than osteoblasts. In the osteocytes isolated from Phex-deficient Hyp mice, the expressions of Dmp1, Fam20c and Pit1 as well as that of Fgf23 were up-regulated. 24-hour treatment with 1,25-dihydroxyvitamin D markedly increased the expression of Fgf23. On the other hand, 24-hour treatment with high phosphate did not alter the expression of Fgf23, although it markedly increased the expression of Dmp1. These results suggest that chronic phosphate load is required for the increase of FGF23 production.
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[Journal Article] Dysregulated gene expression in the primary osteoblasts and osteocytes isolated from hypophosphatemic Hyp mice2014
Author(s)
Miyagawa K, Yamazaki M, Kawai M, Nishino J, Koshimizu T, Ohata Y, Tachikawa K, Mikuni-Takagaki Y, Kogo M, Ozono K, Michigami T
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Journal Title
PLoS One
Volume: 9
Pages: e93840
DOI
Peer Reviewed
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[Journal Article] Elevated fibroblast growth factor 23 exerts its effects on placenta and regulates vitamin D metabolism in pregnancy of Hyp mice2014
Author(s)
Ohata Y, Yamazaki M, Kawai M, Tsugawa N, Tachikawa K, Koinuma T, Miyagawa K, Kimoto A, Nakayama M, Namba N, Yamamoto H, Okano T, Ozono K, Michigami T
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Journal Title
J Bone Miner Res
Volume: (E-pub)
DOI
Peer Reviewed
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[Journal Article] Retention of fetuin-A in renal tubular lumen protects the kidney from nephrocalcinosis in rats2013
Author(s)
Matsui I, Hamano T, Mikami S, Inoue K, Shimomura A, Nagasawa Y, Michigami T, Ohnishi T, Fujii N, Nakano C, Kusunoki Y, Kitamura H, Iwatani H, Takabatake Y, Kaimori JY, Matsuba G, Okoshi K, Kimura-Suda H, Tsubakihara Y, Rakugi H, Isaka Y
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Journal Title
Am J Physiol Renal Physiol
Volume: 304
Pages: F751–760
DOI
Peer Reviewed
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[Presentation] Fetal stage-specific mineral metabolism in Hyp mice is associated with effects of FGF23 on placenta2013
Author(s)
Ohata Y, Yamazaki M, Kawai M, Tachikawa K, Koinuma T, Miyagawa K, Kimoto A, Nakayama M, Namba N, Yamamoto H, Ozono K, Michigami T
Organizer
35th Annual Meeting of the American Society for Bone and Mineral Research
Place of Presentation
Bartimore
Year and Date
20131004-07
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