2013 Fiscal Year Final Research Report
Effects of transplantation of neural crest cells derived from iPS cells on diabetic polyneuropathy in mice
| Project/Area Number |
23591303
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Aichi Medical University |
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Principal Investigator |
NAKAMURA Jiro 愛知医科大学, 医学部, 教授 (40283444)
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| Co-Investigator(Kenkyū-buntansha) |
ISOBE Kennichi 名古屋大学, 医学系研究科, 教授 (20151441)
KAMIYA Hideki 愛知医科大学, 医学部, 准教授 (70542679)
NARUSE Keiko 愛知学院大学, 歯学部, 准教授 (30387576)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 糖尿病性多発神経障害 / 細胞移植 / 人工多能性幹細胞 / 神経堤細胞 |
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| Research Abstract |
Recent studies have shown that transplantation of neural crest cells (NCCs) is effective on spinal cord injury. Here we used NCCs from aged mouse iPS cells for treatment of diabetic polyneuropathy. iPS cells were differentiated on PA6 cells with BMP4 , and p75 positive cells were defined as NCCs. NCCs were transplanted into hind limb skeletal muscles of STZ-diabetic mice. Transplanted NCCs differentiated into vascular smooth muscle cells or Schwann cells in plantar skin or soleus muscle. Impaired nerve functions in diabetic mice were ameliorated by the transplantation of NCCs. In addition, transplantation of NCCs increased intraepidermal nerve fiber densities, plantar skin blood flow and capillaries in DM. These results suggest that transplantation of NCCs derived from iPS cells could have therapeutic effects on DPN through paracrine actions of growth factors secreted by NCCs and differentiation of NCCs into Schwann cells and vascular smooth muscle cells.
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[Journal Article] Transplantation of neural crest like cells derived from induced pluripotent stem cells improves diabetic polyneuropathy in mice2013
Author(s)
Okawa T, Kamiya H, Himeno T, Kato J, Seino Y, Fujiya A, Kondo M, Tsunekawa S, Naruse K, Hamada Y, Ozaki N, Cheng Z, Kito T, Suzuki H, Ito S, Nakamura J, Isobe K
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Journal Title
Cell Transplant
Volume: 22
Pages: 1763-1783
Peer Reviewed
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[Journal Article] Mesenchymal stem cell-like cells derived from mouse induced pluripotent stem cells ameliorate diabetic polyneuropathy in mice2013
Author(s)
Himeno T, Kamiya H, Naruse K, Cheng Z, Ito S, Kondo M, Okawa T, Fujiya A, Kato J, Suzuki H, Kito T, Hamada Y, Oiso Y, Isobe K, Nakamura J
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Journal Title
DOI
Peer Reviewed
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[Journal Article] Beneficial effects of exendin-4 on experimental polyneuropathy in diabetic mice2011
Author(s)
Himeno T, Kamiya H, Naruse K, Ozaki N, Seino Y, Shibata T, Kondo M, Kato J, Okawa T, Fukami A, Hamada Y, Inagaki N, Seino Y, Drucker D. J, Oiso Y, Nakamura J
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Journal Title
Diabetes
Volume: 60
Pages: 2387-2406
Peer Reviewed
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[Journal Article] Transplantation of bone marrow-derived mononuclear cells improves mechanical hyperalgesia, cold allodynia and nerve function in diabetic neuropathy2011
Author(s)
Naruse K, Sato J, Funakubo M, Hata M, Nakamura N, Kobayashi Y, Kamiya H, Shibata T, Kondo M, Himeno T, Matsubara T, Oiso Y, Nakamura J
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Journal Title
PLoS ONE
Volume: 6
Pages: e27458
Peer Reviewed
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