2013 Fiscal Year Final Research Report
Analysis of myeloma stem cells and their niche
| Project/Area Number |
23591404
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | Kyoto Pharmaceutical University (2012-2013)
Kyoto Prefectural University of Medicine (2011) |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIRAI Hideyo 京都大学, 大学院医学研究科, 助教 (50315933)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 多発性骨髄腫 / 低酸素 / 骨髄微小環境 / がん幹細胞 |
|---|
| Research Abstract |
We investigated the characteristics of hypoxia-adapted MM (HA-MM) cells. Irradiated NOD/SCID mice were inoculated with AMO-1 MM cells. After 2 or 4 weeks transplantation, the inoculated MM cells were positive for pimonidazole. These observations suggested that MM cells are hypoxic in the BM. We then established HA-MM cell lines that can continue to proliferate in hypoxic conditions (1% O2) for more than six months. The G0 fraction cells significantly increased in HA-MM cells compared with those in the parental MM cells. The survival durations of mice transplanted with HA-MM cells were significantly shorter than that of mice transplanted with parental MM cells. Stem cell markers such as Sox2, Oct3, and Nanog mRNA transcripts increased in the HA-MM AMO-1 cells. Furthermore, phosphorylated Smad2 expression was increased in HA-AMO-1 cells. These findings suggest that HA-MM cells possess stem cell-like characters and may provide a useful model to investigate the mechanism of MM stem cells.
|
Research Products
(40 results)
