2013 Fiscal Year Final Research Report
Analysis of the novel FcgR transmembrane interface motif displaying a polymorphism associate with human SLE susceptibility
| Project/Area Number |
23591459
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Ochanomizu University (2012-2013)
The University of Tokyo (2011) |
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Principal Investigator |
HONDA Zen-ichiro お茶の水女子大学, 保健管理センター, 教授 (70238814)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Hiroaki 広島大学, 原爆放射線医科学研究所, 教授 (40245064)
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| Project Period (FY) |
2011 – 2013
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| Keywords | Fc受容体 / 膜貫通部位 / 遺伝子多型 / シグナル伝達 |
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| Research Abstract |
We have prepared 28 Cys mutants spanning the transmembrane domain of FcgRIIA, stably expressed in human B cells, and the cells were analyzed for Cys crosslinking under unligated, and ligated conditions. We identified unexpectedly complicated TMD assembly and reassembly mechanisms under resting, and activated conditions, and these structural alterations are all responsible for the signal stabilization, and the signal activation. We extrapolated the findings in FcgRIIA to FcgRIIB I232T autoimmune disease promoting polymorphism, and found that the amino acid substitution does alter the mode of the TMD association.
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Research Products
(5 results)
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[Journal Article] CIZ1, a p21^<Cip1/Waf1>-interacting protein, functions as a tumor suppressor in vivo2013
Author(s)
Nishibe R, Watanabe W, Ueda T, Yamasaki N, Koller R, Wolff L, Honda Z-i, Ohtsubo M, Honda H
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Journal Title
FEBS Lett
Volume: 587
Pages: 1529-1535
DOI
Peer Reviewed
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[Journal Article] Hemp, an mbt domain-containing protein, plays essential roles in hematopoietic stem cell function and skeletal formation2011
Author(s)
Honda H, Takubo K, Oda H, Kosaki K, Tazaki T, Yamasaki N, Miyazaki K, Moore KA, Honda Zi, Suda T, Lemischka IR
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Journal Title
Proc Natl Acad Sci USA
Volume: 108
Pages: 2468-2473
DOI
Peer Reviewed
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