Immunological effects of Ureaplasma parvum on pregnancy outcome in mice.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591609
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Research Institute, Osaka Medical Center for Maternal and Child Health
• Principal Investigator: ITARU Yanagihara 地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), その他部局等, その他 (60314415)
• Co-Investigator(Kenkyū-buntansha): NAKAHIRA Kumiko 地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), 免疫部門, 流動研究員 (20581317) ; NISHIUMI Fumiko 地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), 免疫部門, 流動研究員 (60599596)
• Project Period (FY): 2011 – 2013
• Keywords: 早産 / 周産期感染症 / ウレアプラズマ / 病原因子 / ゲノム解析

Research Abstract

Ureaplasma spp. are members of the family Mycoplasmataceae and have been considered to be associated with chorioamnionitis and preterm delivery. We purified multiple-banded antigen (MBA) from U. parvum, and synthesized N-terminal diacylated lipopeptide of MBA, UPM-1. Using luciferase assays, both purified MBA and UPM-1 activated the NF-kB pathway via TLR2 in vitro. The intrauterine MBA injection on day 15 of gestation on pregnant C3H/HeN mice group had a significantly higher incidence of intrauterine fetal death (IUFD; 38.5%). Intrauterine injection of UPM-1 caused preterm deliveries (80.0%). The placentas of the UPM-1 injection group showed neutrophil infiltration. We also reported the complete genome sequence of Ureaplasma parvum serovar 3, clinical strain SV3F4, isolated from a Japanese patient with a history of an infectious abortion.

Research Products

• [Journal Article] Complete Genome Sequence of Ureaplasma parvum Serovar 3 Strain SV3F4, Isolated in Japan (2014)
  • Author(s): Wu HN, Nakura Y, Motooka D, Nakamura S, Nishiumi F, Ishino S, Kawai Y, Tanaka T, Takeuchi M, Nakayama M, Fujita T, Yanagihara I
  • Journal Title: Genome Announc Volume: 2(3)
  • DOI: 10.1128/genomeA.00256-14
  • Peer Reviewed
• [Journal Article] Effects of Ureaplasma parvum lipoprotein multiple-banded antigen on pregnancy outcome in mice (2013)
  • Author(s): Uchida K, Nakahira K, Mimura K, Shimizu T, De Seta F, Wakimoto T, Kawai Y, Nomiyama M, Kuwano K, Guaschino S, Yanagihara I
  • Journal Title: J Reprod Immunol Volume: 100(2) Pages: 118-27
  • DOI: 10.1016/j.jri.2013.10.001
  • Peer Reviewed
• [Journal Article] Phosphorylation of FOXP3 by LCK downregulates MMP9 expression and represses cell invasion (2013)
  • Author(s): Nakahira K, Morita A, Kim NS, Yanagihara I
  • Journal Title: PLoS One Volume: 8(10) Pages: e77099
  • DOI: 10.1371/journal.pone.0077099
  • Peer Reviewed
• [Journal Article] Structural basis ofα-catenin recognition by EspB from enterohaemorrhagic E. coli based on hybrid strategy using low-resolution structural and protein dissection (2013)
  • Author(s): Hamaguchi M, Kamikubo H, Suzuki KN, Hagihara Y, Yanagihara I, Sakata I, Kataoka M, Hamada D
  • Journal Title: PLoS One Volume: 8(8) Pages: e71618
  • DOI: 10.1371/journal.pone.0071618
  • Peer Reviewed
• [Journal Article] An Arthrobacter spp. bacteremia leading to fetal death and maternal disseminated intravascular coagulation (2013)
  • Author(s): Shigeta N, Ozaki K, Hori K, Ito K, Nakayama M, Nakahira K, Yanagihara I
  • Journal Title: Fetal Pediatr Pathol Volume: 31(1) Pages: 25-31
  • DOI: 10.3109/15513815.2012.659413
  • Peer Reviewed
• [Journal Article] Establishment and characterization of Roberts syndrome and SC phocomelia model medaka (Oryzias latipes) (2012)
  • Author(s): Morita A, Nakahira K, Hasegawa T, Uchida K, Taniguchi Y, Takeda S, Toyoda A,Sakaki Y, Shimada A, Takeda H, Yanagihara I
  • Journal Title: Dev Growth Differ Volume: 54(5) Pages: 588-604
  • DOI: 10.1111/j.1440-169X.2012.01362.x
  • Peer Reviewed
• [Journal Article] E2A and CBP/p300 act in synergy to promote chromatin accessibility of the immunoglobulinκlocus (2012)
  • Author(s): Sakamoto S, Wakae K, Anzai Y, Murai K, Tamaki N, Miyazaki M, Miyazaki K,Romanow WJ, Ikawa T, Kitamura D, Yanagihara I, Minato N, Murre C, Agata Y
  • Journal Title: J Immunol Volume: 188(11) Pages: 5547-60
  • DOI: 10.4049/jimmunol.1002346
  • Peer Reviewed
• [Journal Article] Annexin A2 in amniotic fluid : correlation with histological chorioamnionitis, preterm premature rupture of membranes, and subsequent preterm delivery (2012)
  • Author(s): Namba F, Ina S, Kitajima H, Yoshio H, Mimura K, Saito S, Yanagihara I
  • Journal Title: J Obstet Gynaecol Res Volume: 38(1) Pages: 137-44
  • DOI: 10.1111/j.1447-0756.2011.01651.x
  • Peer Reviewed
• [Journal Article] RodZ regulates the post-transcriptional processing of the Shigella sonnei typeIII secretion system (2011)
  • Author(s): Mitobe J, Yanagihara I, Ohnishi K, Yamamoto S, Ohnishi M, Ishihama A, Watanabe H
  • Journal Title: EMBO Rep Volume: 12(9) Pages: 911-6
  • DOI: 10.1038/embor.2011.132
  • Peer Reviewed
• [Journal Article] Ceftriaxone preconditioning confers neuroprotection in neonatal rats through glutamate transporter 1 upregulation (2011)
  • Author(s): Mimura K, Tomimatsu T, Minato K, Jugder O, Kinugasa-Taniguchi Y, Kanagawa T, Nozaki M, Yanagihara I, Kimura T
  • Journal Title: Reprod Sci Volume: 18(12) Pages: 1193-201
  • DOI: 10.1177/1933719111410710
  • Peer Reviewed
• [Journal Article] Silica and titanium dioxide nanoparticles cause pregnancy complications in mice (2011)
  • Author(s): Yamashita K, Yoshioka Y, Higashisaka K, Mimura K, Morishita Y, Nozaki M, Yoshida T, Ogura T, Nabeshi H, Nagano K, Abe Y, Kamada H, Monobe Y, Imazawa T,Aoshima H, Shishido K, Kawai Y, Mayumi T, Tsunoda S, Itoh N, Yoshikawa T,Yanagihara I, Saito S, Tsutsumi Y
  • Journal Title: Nat Nanotechnol Volume: 6(5) Pages: 321-8
  • DOI: 10.1038/nnano.2011.41
  • Peer Reviewed
• [Journal Article] Regulation of TCR Vγ2 gene rearrangement by the helix-loop-helix protein, E2A (2011)
  • Author(s): Nozaki M, Wakae K, Tamaki N, Sakamoto S, Ohnishi K, Uejima T, Minato N, Yanagihara I, Agata Y
  • Journal Title: Int Immunol Volume: 23(5) Pages: 297-305
  • DOI: 10.1093/intimm/dxr005
  • Peer Reviewed
• [Journal Article] Relationship between heat-induced fibrillogenicity and hemolytic activity of thermostable direct hemolysin and a related hemolysin of Vibrio parahaemolyticus (2011)
  • Author(s): Ohnishi K, Nakahira K, Unzai S, Mayanagi K, Hashimoto H, Shiraki K, Honda T, Yanagihara I
  • Journal Title: FEMS Microbiol Lett Volume: 318(1) Pages: 10-7
  • DOI: 10.1111/j.1574-6968.2011.02233.x
  • Peer Reviewed
• [Journal Article] Polymorphisms in the annexin A5 gene promoter in Japanese women with recurrent pregnancy loss (2011)
  • Author(s): Miyamura H, Nishizawa H, Ota S, Suzuki M, Inagaki A, Egusa H, Nishiyama S,Kato T, Pryor-Koishi K, Nakanishi I, Fujita T, Imayoshi Y, Markoff A, Yanagihara I, Udagawa Y, Kurahashi H
  • Journal Title: Mol Hum Reprod Volume: 17(7) Pages: 447-52
  • DOI: 10.1093/molehr/gar008
  • Peer Reviewed
• [Presentation] 胎児炎症反応症候群(FIRS)の病態と微生物 (2013)
  • Author(s): 柳原格
  • Organizer: 第58回日本未熟児新生児学会・学術集会
  • Place of Presentation: 金沢(シンポジウム)
  • Year and Date: 20131130-1202
• [Presentation] ウレアプラズマ陽性母体児の臨床的検討 (2013)
  • Author(s): 江頭政和、飯田千晶、七條了宣、江頭智子、水上朋子、松尾幸司、高柳俊光、柳原格
  • Organizer: 第58回日本未熟児新生児学会・学術集会
  • Place of Presentation: 金沢
  • Year and Date: 20131130-1202
• [Presentation] 流早産原因菌ウレアプラズマの病態解明 (2013)
  • Author(s): 柳原格
  • Organizer: 第21回日本胎盤学会学術集会シンポジウム「炎症と胎盤
  • Place of Presentation: 名古屋(シンポジウム)
  • Year and Date: 20131025-26
• [Presentation] 細胞を用いたTLR2を介するNF-κB転写活性に対するプロゲステロン、フラーレンの抑制効果 (2013)
  • Author(s): 脇本哲、味村和哉、内田薫、金川武司、木村正、柳原格
  • Organizer: 第49回日本周産期・新生児医学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20130714-16
• [Presentation] Ureaplasma parvumの細胞内動態解析 (2013)
  • Author(s): 西海史子、柳原格
  • Organizer: 第40回日本マイコプラズマ学会学術集会
  • Place of Presentation: 秋葉原
  • Year and Date: 20130523-24
• [Presentation] 薬剤耐性ウレアプラズマの同定 (2013)
  • Author(s): 名倉由起子、河合泰宏、萬野和、藤田富雄、呉恒寧、柳原格
  • Organizer: 第40回日本マイコプラズマ学会学術集会
  • Place of Presentation: 秋葉原
  • Year and Date: 20130523-24
• [Presentation] Structural aspects of EspA and EspB proteins of entetohaemorrhagic E. coli (2013)
  • Author(s): Itaru yanagihara, kumiko nakahira
  • Organizer: 日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本国内総会
  • Place of Presentation: 京都
  • Year and Date: 2013-08-08
• [Presentation] 非晶質ナノシリカの細胞膜への結合とナノ生殖毒性との関連 (2012)
  • Author(s): 小野寺章、西海史子、古田拓也、中平久美子、石井幸奈、本間安季、太田舞子、諸澤瑛、福井健太郎、米村重信、柳原格、堤康央、河合裕一
  • Organizer: 第85回日本生化学会
  • Place of Presentation: 福岡
  • Year and Date: 20121214-16
• [Presentation] The aggregative property of thermostable direct hemolysin (2012)
  • Author(s): Itaru Yanagihara, Kumiko Nakahira
  • Organizer: United States-Japan Cooperative Medical Science Program
  • Place of Presentation: Chiba
  • Year and Date: 20121212-14
• [Presentation] Obstetric complication-associated ANXA5 promoter polymorphisms affect gene expression via DNA secondary structures (2012)
  • Author(s): Hideto Inagaki, Sayuri Ota, Haruki Nishizawa, Hironori Miyamura, Kumiko Nakahira, Macjiko Suzuki, Sachie Nishiyama, Yasuhiro Udagawa, Itaru Yanagihara, Hiroki Kurahashi
  • Organizer: American Society of Human Genetics
  • Place of Presentation: san Francisco
  • Year and Date: 20121106-10
• [Presentation] 習慣流産をおこすANXA5上流のグアニン四重鎖構造多型 (2012)
  • Author(s): 太田小百合、稲垣秀人、宮村浩徳、西澤春紀、中平久美子、柳原格、宇田川康博、倉橋浩樹
  • Organizer: 人類遺伝学
  • Place of Presentation: 東京
  • Year and Date: 20121025-27
• [Presentation] Massive intervillous fibrin deposition in women with recurrent pregnancy loss (2012)
  • Author(s): Misako Iwata, Msahiro Nakayama, Masako Waguri, Nobuaki Mitsuda, Itaru Yanagihara, Tomio Fujita, Isao Nakanishi
  • Organizer: International Federation of Placenta Assocications
  • Place of Presentation: Hiroshima
  • Year and Date: 20120918-21
• [Presentation] 出生直後の新生児胃液中顆粒球エラスターゼ、サイトカインは組織学的絨毛膜羊膜炎を反映する (2012)
  • Author(s): 木下大介、玉置祥子、斉藤広幸、川村彬子、酒井絵美子、井石倫弘、甲斐明彦、前川周、隅清彰、中山雅弘、柳原格
  • Organizer: 周産期新生児学会
  • Place of Presentation: 大宮
  • Year and Date: 20120708-10
• [Presentation] 早産における臨床分離マイコプラズマ科の血清型診断のまとめ (2012)
  • Author(s): 名倉由起子、内田薫、柳原格
  • Organizer: 周産期新生児学会
  • Place of Presentation: 大宮
  • Year and Date: 20120708-10
• [Presentation] Ureaplasma parvumの細胞内取り込み機構・細胞内動態解析 (2012)
  • Author(s): 西海史子、柳原格
  • Organizer: 日本マイコプラズマ学会
  • Place of Presentation: 盛岡
  • Year and Date: 20120524-25
• [Presentation] Gastric fluid cytokines in preterm infants : a useful test for evaluating antenatal inflammation (2012)
  • Author(s): Daisuke Kinoshita, Kiyoaki Sumi, Hiroaki Tubouchi, Kensi Wasada, Kumiko Nakahira, Itaru Yanagihara
  • Organizer: 17^<th> Congress of the Federation of Asian and Oceania Perinatal Societies (FAOPS) and the 16th Annual Congress of the Perinatal Society of Australia and New Zealand (PSANZ)–FAOPS & PSANZ
  • Place of Presentation: Sydney
  • Year and Date: 20120318-21
• [Presentation] 不育症患者におけるアネキシンA5遺伝子SNP5と生児獲得に関する研究 (2011)
  • Author(s): 岩田みさ子、中平久美子、和栗雅子、光田信明、倉橋浩樹、柳原格、中西功
  • Organizer: 第56回日本生殖医学会
  • Place of Presentation: 横浜
  • Year and Date: 20111208-09
• [Presentation] ANXA5プロモーター領域のグアニン四重鎖構造と習慣流産 (2011)
  • Author(s): 太田小百合、稲垣秀人、宮村浩徳、西澤春紀、柳原格、宇田川康博、倉橋浩樹
  • Organizer: 第56回日本人類遺伝学会
  • Place of Presentation: 千葉
  • Year and Date: 20111109-12
• [Presentation] Inflammatory response and intrauterine fetal death in pregnant mice induced by MBA from clinical isolate of Ureaplasma parvum (2011)
  • Author(s): Kaoru Uchida, Kumiko Nakahira, Takashi Shimizu and Itaru Yanagihara
  • Organizer: Joint Congress of The 5^<th> Meeting of Asian Organization for Mycoplasmology. The 38^<th> Meeting of the Japanese Society of Mycoplasmology
  • Place of Presentation: Nagasaki
  • Year and Date: 20111019-21
• [Presentation] Virulence factorsofUreaplasma parvum (2011)
  • Author(s): Itaru Yanagihara, Kaoru Uchida, Kumiko Nakahira and Fumiko Nishiumi
  • Organizer: Joint Congress of The 5^<th> Meeting of Asian Organization for Mycoplasmology. The 38^<th> Meeting of the Japanese Society of Mycoplasmology
  • Place of Presentation: Nagasaki
  • Year and Date: 20111019-21
• [Presentation] 流早産関連細菌Ureaplasma parvumの病原因子の同定 (2011)
  • Author(s): 内田薫、味村和哉、柳原格
  • Organizer: 第47回日本周産期・新生児医学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 20110710-12
• [Presentation] ナノシリカの妊娠マウス投与が胎盤に与える影響 (2011)
  • Author(s): 味村和哉、野崎昌俊、柳原格
  • Organizer: 第47回日本周産期・新生児医学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 20110710-12
• [Presentation] 流早産起因微生物ウレアプラズマとその病原発揮機構 (2011)
  • Author(s): 柳原格、中平久美子、内田薫
  • Organizer: 第58回トキシンシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20110706-07
• [Presentation] Nanoparticle can cross the placental barrier in pregnant mice (2011)
  • Author(s): 柳原格、味村和哉、名倉由起子、西海史子、中平久美子
  • Organizer: 日米コレラ
  • Place of Presentation: 京都
  • Year and Date: 2011-08-03
• [Book] 腸炎ビブリオ第IV集VIII-2「耐熱性溶血毒の構造学的解析」
  • Author(s): 柳原格、中平久美子
  • Total Pages: 277-286
  • Publisher: 近代出版
• [Remarks]
  • URL: http://www.mch.pref.osaka.jp/research/developmental/index.html