2014 Fiscal Year Final Research Report
The development of the method of producing cultured skin using adipose tissue-derived mesenchymal stem cells.
Project/Area Number |
23591647
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Dermatology
|
Research Institution | Ehime University |
Principal Investigator |
KENJI Kameda 愛媛大学, 総合科学研究支援センター, 講師 (60363264)
|
Co-Investigator(Kenkyū-buntansha) |
SHIRAKATA Yuji 愛媛大学, 大学院医学系研究科, 准教授 (50226320)
|
Project Period (FY) |
2011-04-28 – 2015-03-31
|
Keywords | 再生医療 / 三次元培養 / 脂肪細胞 / 幹細胞 / 角化細胞 |
Outline of Final Research Achievements |
We cultured adiposed-derived mesenchymal stem cells (ASCs), but cytokeratin-positive cells could not be detected. Therefore we seeded and gelled ASCs with Type I collagen solutions on the culture inserts, and then added the appropriate amount of 10 % FCS/DMEM inside and outside the inserts. And then were plated onto the gel cultures were stratified perform air exposure. We demonstrated three-dimensional configuration of the skin with HE staining. Three-dimensional reconstruction of the skin was subjected to HE staining was confirmed. It shows that ASCs can function as an alternative to dermal fibroblasts.
|
Free Research Field |
分子生物学
|