2013 Fiscal Year Final Research Report
Intestinal epithelial culture under an air-liquid interface
| Project/Area Number |
23591857
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Gunma University |
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Principal Investigator |
YOKOBORI Takehiko 群馬大学, 医学(系)研究科(研究院), 助教 (60420098)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Tatsuya 群馬大学, 医学部附属病院, 助教 (70372349)
TANAKA Naritake 群馬大学, 医学部附属病院, 助教 (30546726)
KUWANO Hiroyuki 群馬大学, 大学院医学系研究科, 教授 (90186560)
INOSE Takenori 群馬大学, 医学部, 助教 (90609968)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 食道 / 再生 / 培養 |
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| Research Abstract |
Our study led to two new findings: (1) The intestinal epithelial culture method of Ootani et al. (Nat Med. 2009 Jun;15(6):701-706) can be applied to mouse and human esophageal cultures. (2) Esophageal organoids are rod-like luminal structures lined with fibroblasts, and regeneration of the esophageal mucosal surface can be almost completely achieved in vitro.
The method described in the generation of long-term cultures of human esophageal cells without the need for gene transfer for immortalization. Due to autologous myofibroblasts from a collected specimen are used as a niche, feeder cells are not required for plating. In addition, the matrix gel, in which cells are embedded, and the culture medium are commercially available. These merits are important for clinical application of our method.Therefore, we believe that this new method has the potential to be an important tool to advance research in intestinal biology, carcinogenesis, and regenerative medicine in both mice and humans.
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[Journal Article] FBXW7 mediates chemotherapeutic sensitivity and prognosis in NSCLCs2014
Author(s)
Yokobori T, Yokoyama Y, Mogi A, Endoh H, Altan B, Kosaka T, Yamaki E, Yajima T, Tomizawa K, Azuma Y, Onozato R, Miyazaki T, Tanaka S, Kuwano H
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Journal Title
Mol Cancer Res
Volume: 12(1)
Pages: 32-7
Peer Reviewed
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[Journal Article] Nuclear karyopherin-α2 expression in primary lesions and metastatic lymph nodes was associated with poor prognosis and progression in gastric cancer2013
Author(s)
Altan B, Yokobori T, Mochiki E, Ohno T, Ogata K, Ogawa A, Yanai M, Kobayashi T, Luvsandagva B, Asao T, Kuwano H
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Journal Title
Carcinogenesis
Volume: 34(10)
Pages: 2314-21
Peer Reviewed
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[Journal Article] MiR-150 is associated with poor prognosis in esophageal squamous cell carcinoma via targeting the EMT inducer ZEB12013
Author(s)
Yokobori T, Suzuki S, Tanaka N, Inose T, Sohda M, Sano A, Sakai M, Nakajima M, Miyazaki T, Kato H, Kuwano H
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Journal Title
Cancer Sci
Volume: 104(1)
Pages: 48-54
Peer Reviewed
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[Journal Article] Copy number loss of FBXW7 is related to gene expression and poor prognosis in esophageal squamous cell carcinoma2012
Author(s)
Yokobori T, Mimori K, Iwatsuki M, Ishii H, Tanaka F, Sato T, Toh H, Sudo T, Iwaya T, Tanaka Y, Onoyama I, Kuwano H, Nakayama KI, Mori M
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Journal Title
Int J Oncol
Volume: 41(1)
Pages: 253-9
Peer Reviewed
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